Objective PrPc binds copper and has antioxidant activity enhancing the survival of cells in culture, suggesting that PrPSc accumulation may lead to an aberrant copper metabolism altering the cellular redox state. This proposal centres on understanding to what extend PrPc expression, and susceptibility to prion can be influenced by oxidative stress. In vitro studies with murine neuroblastoma N2a and Schwann MSC80A cells, and in vivo studies with transgenic mice over expressing either mice, human or bovine PrPc, under normal conditions or after various treatments (irradiation, hypoxia, Cu depletion and Mn feeding, ingestion of phosmet) known to cause oxidative stress, will show if and to what extent these stress situations may enhance PrPc expression and the susceptibility to prion infection. The work will also contribute to the basic understanding of PrP and may give information or cellular tools (new chronically infected non neuronal cell model) that could benefit treatment. Fields of science medical and health scienceshealth sciencesnutrition Programme(s) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Topic(s) 1.1.1.-9. - Neurosciences Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator UNIVERSITE JOSEPH FOURIER - GRENOBLE 1 Address Domaine de la merci - faculté de médecine 38706 La tronche France See on map EU contribution € 0,00 Participants (5) Sort alphabetically Sort by EU Contribution Expand all Collapse all CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE France EU contribution € 0,00 Address Domaine de la merci - faculté de médecine 38706 La tronche See on map CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE France EU contribution € 0,00 Address Rue de la ferollerie, 3b 45071 Orleans See on map UNIVERSIDAD DE ZARAGOZA Spain EU contribution € 0,00 Address Miguel servet, 177 50013 Zaragoza See on map UNIVERSITY OF ROME "LA SAPIENZA" Italy EU contribution € 0,00 Address Piazzale aldo moro, 5 00185 Roma See on map UNIVERSITÀ DEGLI STUDI - L'AQUILA Italy EU contribution € 0,00 Address Via vetoio 67010 Coppito-l'aquila See on map
