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Functional properties, bioactivities and bioavailability of phytochemicals, especially anthocyanins, form processed foods

Objective

Bacteria posses an amazing capacity for secreting extracellular and cell surface proteins and, thus, are potentially ideally-suited for the production of heterologous proteins of industrial relevance (remedial and other enzymes, single chain antibodies, antigens, etc.) Surprisingly, little effort has been devoted to evaluate the respective merits of the many different secretion systems that bacteria posses, and only a limited number of systems have been studied. We will rectify this situation by developing tools to measure "secretion potential" under uniform, controlled conditions (epitome tagging, antibody recognition, enzymatic activity, immunocytology and ELISA assays) and to evaluate 6 representative secretion systems with respect to the several criteria, including yields, ease of manipulation, ability to accept passenger proteins and correct protein folding. Intra-consortium cross-evaluation will the data and pinpoint systems of particular merit that will be optimised and developed into manageable gene cloning and expression packages.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

UNIVERSITY OF HELSINKI
Address
Yliopistonkatu 4
00014 Helsinki
Finland

Participants (7)

Christian-Albrechts-Universität Kiel
Germany
Address
Olshausenstraße 40-60
24118 Kiel
DANISH VETERINARY AND FOOD ADMINISTRATION
Denmark
Address
Moerkhoej Bygade 19
2860 Soeborg
DR. MARCUS GMBH
Germany
Address
Geesthachter Strasse 103
21494 Geesthacht
ROWETT RESEARCH INSTITUTE
United Kingdom
Address
Greenburn Road
AB21 9SB Aberdeen
TECHNICAL UNIVERSITY OF DENMARK
Denmark
Address
Dtu, Blgd. 221
2800 Lyngby
THE SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES
Sweden
Address
10,Arrheniusplan 2C
750 07 Uppsala
UNIVERSITA DEGLI STUDI DI PAVIA
Italy
Address
Corso Strada Nuova 65
27100 Pavia