Bacteria posses an amazing capacity for secreting extracellular and cell surface proteins and, thus, are potentially ideally-suited for the production of heterologous proteins of industrial relevance (remedial and other enzymes, single chain antibodies, antigens, etc.) Surprisingly, little effort has been devoted to evaluate the respective merits of the many different secretion systems that bacteria posses, and only a limited number of systems have been studied. We will rectify this situation by developing tools to measure "secretion potential" under uniform, controlled conditions (epitome tagging, antibody recognition, enzymatic activity, immunocytology and ELISA assays) and to evaluate 6 representative secretion systems with respect to the several criteria, including yields, ease of manipulation, ability to accept passenger proteins and correct protein folding. Intra-consortium cross-evaluation will the data and pinpoint systems of particular merit that will be optimised and developed into manageable gene cloning and expression packages.
Funding SchemeCSC - Cost-sharing contracts
750 07 Uppsala