Coeliac disease (CD) is caused by intolerance to gluten, a common food protein. The disease affects approximately one million Europeans. There is strong evidence that CD is the result of aberrant T cell responses to gluten peptides bound to the disease predisposing HLA-DQ molecules. Recently, we have identified several such peptides. Moreover, T cell recognition of many of these peptides is dependant onthem being enzymatic ally modified in a way that promotes their binding to HLA-DQ.These results provide a rationale for the development of CD. We now propose to:
a) To determine the relative importance of the identified peptides.
b) To demonstrate an in vivo effect of these peptides.
c) To investigate the feasibility of therapeutic intervention.
d) To develop reliable and easy to handle test systems for the detection of toxic gluten peptides in food products.