Detoxified adenylate cyclase toxin: a major improvement for the development of safe, efficient and multipurpose vaccines.

Objective

The cell-invasive and genetically detoxified Aden late cycles toxic (ACT) represents a novel, non-explicative and inherently safe vector for in vivo delivery of vicinal antigens into antigen presenting cells. Experimental delivery of antigens by ACT was shown to result in efficient induction of both prophylactic and therapeutic catatonic CD8 T lymphocyte responses against viruses and tumours in mice, thereby meeting on the current challenges in vaccine development. Our project aims at detailed analysis of the mechanisms underlying the vicinal potential of the ACT vector and evaluations of its versatility as a safe, efficient and polyvalent delivery system for viral (HIV) and tumour antigens. Pre-clinical toxicology assessment and ACT delivery system development will be performed, suitable mussel adjuvant will be identified and rational design of the vector molecule will be initiated, on the basis of a thorough understanding of ACT structure and function. To this end, specialists in immunology, toxicology and vaccine development have joined forces with biochemists, molecular and structural biologists and

Programme(s)

• FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002

Topic(s)

• 1.1.1.-2. - Key action Control of Infectious Diseases

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

Address

Centre Universitaire Paris Sud - Batiment 209 D
91898 Orsay
France

Participants (8)

CHIRON S.R.L.

Italy

Address

Via Fiorentina 1
53100 Siena

CHRISTIAN DE DUVE INSTITUTE OF CELLULAR PATHOLOGY

Belgium

Address

Avenue Hippocrate 74
1200 Bruxelles

INSTITUT PASTEUR

France

Address

Rue Du Docteur Roux 25
75724 Paris

INSTITUTE OF MICROBIOLOGY - ACADEMY OF SCIENCES OF THE CZECH REPUBLIC

Czechia

Address

Videnska 1083
14220 Praha 4

NATIONAL BIOLOGICAL STANDARDS BOARD

United Kingdom

Address

Blanche Lane, South Mimms
EN6 3QG Potters Bar

THE UNIVERSITY OF THE BASQUE COUNTRY

Spain

Address

1397,Barrio Sarriena S/n
48940 Leioa

UNIVERSITY OF GLASGOW

United Kingdom

Address

University Avenue
Glasgow

UNIVERSITY OF WUERZBURG

Germany

Address

Am Hubland
97074 Würzburg