This project will study basic inmunopathological mechanisms, including cellular and cytokine profiles, related to intraperitoneal immune granulomas seen in vaccinated farmed fish Atlantic salmon, rainbow trout and sea bass following the use of oil-adjuvant vaccines containing Aromas salmonicida, Vibrato angular or Pasteur Ella viscid. Emphasis on the contribution of eosinophilic granular cells/mast cells and of pro-inflammatory mediators and cytokines to granule formation. Techniques ranging from histocytoschemistry to gene expressions studies and ex vivo tissue explants will be used. Results contributing to the development of safer vaccine formulations for fish are expected.
The project has shed light on the underlying mechanisms of granuloma formation in vaccinated farmed fish and it has been documented that the characteristics of the vaccine formulation, e.g. extracellular products, antigen content and stability has a bearing on the extent of injection site reactions. It has been shown that different cytokines are produced at the site of injection and that it is also up-regulated in immunocompetent organs like the spleen a few days after vaccination.
Funding SchemeCSC - Cost-sharing contracts
AB9 2TN Aberdeen