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Intraperitoneal immunopathological reactions following vaccination of farmed fish. studies of basic immune mechanisms

Objective

This project will study basic inmunopathological mechanisms, including cellular and cytokine profiles, related to intraperitoneal immune granulomas seen in vaccinated farmed fish Atlantic salmon, rainbow trout and sea bass following the use of oil-adjuvant vaccines containing Aromas salmonicida, Vibrato angular or Pasteur Ella viscid. Emphasis on the contribution of eosinophilic granular cells/mast cells and of pro-inflammatory mediators and cytokines to granule formation. Techniques ranging from histocytoschemistry to gene expressions studies and ex vivo tissue explants will be used. Results contributing to the development of safer vaccine formulations for fish are expected.
The project has shed light on the underlying mechanisms of granuloma formation in vaccinated farmed fish and it has been documented that the characteristics of the vaccine formulation, e.g. extracellular products, antigen content and stability has a bearing on the extent of injection site reactions. It has been shown that different cytokines are produced at the site of injection and that it is also up-regulated in immunocompetent organs like the spleen a few days after vaccination.

Call for proposal

Data not available

Coordinator

NORWEGIAN SCHOOL OF VETERINARY SCIENCE
Address
Ullevaalsveien 72
0033 Oslo
Norway

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Participants (4)

ALPHARMA AS
Norway
Address
Harbitzalleen 3 Skoyen
0212 Oslo

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ASSOCIACAO DO INSTITUTO DE BIOLOGIA MOLECULAR E CELULAR
Portugal
Address
Rua De Campo Alegre, 823
4150 Porto

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UNIVERSIDAD DE LEON
Spain
Address
Avenida De La Facultad 25
24071 Leon

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University of Aberdeen
United Kingdom
Address
2 Tillydrone Avenue
AB9 2TN Aberdeen

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