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Diagnostic and epidemiological markers for tracking of endemic and resurgent european leishmaniasis

Objective

Visceral leishmaniasis is one of the most important vector-borne zoonosis in Southern Europe and lacks reliable, rapid, simple and sensitive typing methods. The current reference typing method, allozyme electrophoresis, is demanding and has low-resolution power for genetic diversity and species taxonomy. We propose to develop new alternative molecular typing methods, based on clade-specific markers determined through DNA-based phylogenetic reconstructions. Phylogenies will be built from DNA sequences of genomic loci exhibiting a range of genetic variation from conserved to highly variable (microsatellites), and will be compared with allozyme-based phylogenies. Molecular typing procedures will be designed to fill the above-mentioned typing requirements. Applicability of the new markers will be evaluated for clinical typing of endemic and imported human and canine infections and for pilot studies of sympatric and allopatric populations, therefore addressing fundamental epidemiological and clinical questions. An electronic database for Leishmania typing data and strain information will be generated, and will be available through the Internet.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE
Address
Keppel Street
London
United Kingdom

Participants (9)

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
France
Address
Rue Auguste Broussonet 163
75794 Paris
HELLENIC PASTEUR INSTITUTE
Greece
Address
Vassilissis Sofias Avenue 127
11521 Athens
HUMBOLDT-UNIVERSITAET BERLIN
Germany
Address
Dorotheenstrasse 96
10098 Berlin
INSTITUTE OF HEALTH CARLOS III
Spain
Address
Km 2.2,Carretera De Majadahonda A Pozuelo Km 2.2
28220 Majadahonda
INSTITUTE OF PARASITOLOGY - ACADEMY OF SCIENCES OF THE CZECH REPUBLIC
Czechia
Address
Branisovska 31
370 05 Ceske Budejovice
INSTITUTE OF TROPICAL MEDICINE PRINCE LEOPOLD
Belgium
Address
155,Nationalestraat 155
2000 Anvers /Antwerpen
UNIVERSIDADE NOVA DE LISBOA
Portugal
Address
Rua De Junqueira 96
1349-008 Lisboa
UNIVERSITE DE MONTPELLIER 1
France
Address
Rue Auguste Broussonet 163
34090 Montpellier
UNIVERSITY OF CRETE
Greece
Address
Voutes
71409 Iraklion