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The use of mycorrhizal fungi in phytoremediation projects

Objective

Gene transfer by liposome-encapsulated DNA has several important advantages over other methods of gene transfer.
1) The method is safe.
2) There is no limit to the encapsulated expression vector size or conformation.
3) The liposomes are non-toxic and non-immunogenic and therefore can be injected repeatedly. Previous studies done by some of the participants indicated that liposomes-encapsulated DNA can mediate gene transfer into internal organs - mainly the liver and lungs, following intravenous injection, and gene transfer into the liver during operation procedure therapy applications will be developed toward clinical practice: A)Gene therapy of liver and lung cancer by IL-2 and interferon gamma-gene transfer (to induce immune reaction against the cancer cells) and combination of chemotherapy and cytokine gene transfer. B)Induction of tolerance to liver allograft by liposome mediated gene transfer of CTLA4-lg (which blocks the activation of the T lymphocytes and therefore the graft rejection into the transplanted liver following the transplantation). C)DNA vaccination against hepatitis C virus by liposome-mediated gene transfer of viral genes into the liver. In each of these applications, local and transient expression of the transferred gene is an advantage since biological activity can be obtained without serious side effects that high level of systemic expression might cause.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Universität Köln
Address
Albert Magnus Platz 1
50923 Köln
Germany

Participants (8)

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
France
Address
Rue Notre Dame Des Pauvres 17
54501 Vandoeuvre-les-nancy
JAGIELLONIAN UNIVERSITY
Poland
Address
Golebia 24
31 007 Krakow
LIMBURGS UNIVERSITAIR CENTRUM
Belgium
Address
Wetenschapspark 1
3590 Diepenbeek
PHYTOBACTER GMBH
Germany
Address
Dorfstrasse 39
17291 Wallmow
PIUS FLORIS BOOMVERZORGING VUGHT BV
Netherlands
Address
Baarzenstraat 17
5262 GD Vught
RESEARCH INSTITUTE FOR SOIL SCIENCE AND AGRICULTURAL CHEMISTRY - HUNGARIAN ACADEMY OF SCIENCES
Hungary
Address
15,Herman Otto Ut 15
1022 Budapest
ROBIN PEPINIERES EARL
France
Address
Le Village
05500 Saint Laurent Du Cros
TRITON UMWELTSCHUTZ GMBH
Germany
Address
Zoerbiger Strasse 24/25
6749 Bitterfeld