The aim of this project is to develop a protein-based subunit vaccine against diseases caused by S. pneumonia by preventing its colonisation of the human nasopharinx. Target antigens will be identified among surface proteins which act as adhesins. The first years will be devoted to the discovery of new adhesins and to determine the degree of their allelic/antigenic variation. The role of putative adhesins, selected via genomics and bioinformatics, will be tested using single and combined knock-out mutants in adhesion assays and in a mouse pharyngeal colonization model. Mouse immunization studies will be performed using purified recombinant proteins and the live vaccine vector S.gordonii. Mucosal delivery will be by the nasal route and by colonization of the upper respiratory tract with the live vector. Protection from colonization and from invasive disease will be evaluated, together with the local and systemic immune responses.
Funding SchemeCSC - Cost-sharing contracts
ST1 5PQQ Stoke-on-trent
581 83 Linkoeping
WC1E 7HX London
1081 HV Amsterdam