This project has been assembled to develop safer biologically contained strains for bioremediation purposes based on then on-pathogenic bacterium Pseudomonas putrid KT2440, which has been recognised as a safe strain for recombinant DNAtechnology by the NIH. For the first time, the availability of the entire genome of this bacterium will allow to establish the scientific basis for a rational design of environrnentally friendly genetically modified microbes (EF-GMM). Therefore, taking advantage of this novel information a fundamental aim of this proposal is to develop a large series of genetic and biochemical tools to target the incorporation of renal in stable sites of the host chromosome, in order to enhance the predictability of the ecological behaviour of the strain in polluted soils or bioreactors. Furthermore, these tools in combination with potent in silicon analyses will allow to establish the complete set of genes involved in the degradation ofbiogenic and xenophobic aromatic compounds that constitutes its derivative potential and is the master key to expand the bioremediation capabilities of this strain. The construction of DNA arrays will allow analysing and predicting how the introduction of foreign catabolic genes affects the global pattern of gene expression under different stress conditions. Finally, to verify commercialisation of the envisaged product ...
Funding SchemeCSC - Cost-sharing contracts
60 744 Poznan