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Content archived on 2024-05-21

Exploiting genomics to engineer an environmentally friendly microorganism for bioremediation purposes

Objective

This project has been assembled to develop safer biologically contained strains for bioremediation purposes based on then on-pathogenic bacterium Pseudomonas putrid KT2440, which has been recognised as a safe strain for recombinant DNAtechnology by the NIH. For the first time, the availability of the entire genome of this bacterium will allow to establish the scientific basis for a rational design of environrnentally friendly genetically modified microbes (EF-GMM). Therefore, taking advantage of this novel information a fundamental aim of this proposal is to develop a large series of genetic and biochemical tools to target the incorporation of renal in stable sites of the host chromosome, in order to enhance the predictability of the ecological behaviour of the strain in polluted soils or bioreactors. Furthermore, these tools in combination with potent in silicon analyses will allow to establish the complete set of genes involved in the degradation ofbiogenic and xenophobic aromatic compounds that constitutes its derivative potential and is the master key to expand the bioremediation capabilities of this strain. The construction of DNA arrays will allow analysing and predicting how the introduction of foreign catabolic genes affects the global pattern of gene expression under different stress conditions. Finally, to verify commercialisation of the envisaged product ...

Fields of science (EuroSciVoc)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

CSC - Cost-sharing contracts

Coordinator

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
EU contribution
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Address
144,Velázquez 144
28071 MADRID
Spain

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Total cost

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Participants (7)

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