Objective This project has been assembled to develop safer biologically contained strains for bioremediation purposes based on then on-pathogenic bacterium Pseudomonas putrid KT2440, which has been recognised as a safe strain for recombinant DNAtechnology by the NIH. For the first time, the availability of the entire genome of this bacterium will allow to establish the scientific basis for a rational design of environrnentally friendly genetically modified microbes (EF-GMM). Therefore, taking advantage of this novel information a fundamental aim of this proposal is to develop a large series of genetic and biochemical tools to target the incorporation of renal in stable sites of the host chromosome, in order to enhance the predictability of the ecological behaviour of the strain in polluted soils or bioreactors. Furthermore, these tools in combination with potent in silicon analyses will allow to establish the complete set of genes involved in the degradation ofbiogenic and xenophobic aromatic compounds that constitutes its derivative potential and is the master key to expand the bioremediation capabilities of this strain. The construction of DNA arrays will allow analysing and predicting how the introduction of foreign catabolic genes affects the global pattern of gene expression under different stress conditions. Finally, to verify commercialisation of the envisaged product ... Fields of science natural sciencesbiological sciencesmicrobiologybacteriologynatural scienceschemical sciencesorganic chemistryaromatic compoundsnatural scienceschemical sciencesinorganic chemistrymetalloidsnatural sciencesbiological sciencesgeneticschromosomesnatural sciencesbiological sciencesgeneticsgenomes Programme(s) FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002 Topic(s) 1.1.1.-3. - Key action The "Cell factory" Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS Address 144,velázquez 144 28071 Madrid Spain See on map EU contribution € 0,00 Participants (7) Sort alphabetically Sort by EU Contribution Expand all Collapse all BIOINFOBAK INSTITUTE Poland EU contribution € 0,00 Address Limanowskiego, 24/a 60 744 Poznan See on map HANNOVER MEDICAL SCHOOL Germany EU contribution € 0,00 Address Carl-neuberg-strasse 1 30625 Hannover See on map MINRAV INFRASTRUCTURES 1993 (LTD) Israel EU contribution € 0,00 Address Kiriat-moria 91291 Jerusalem See on map NEWBIOTECHNIC S.A. Spain EU contribution € 0,00 Address Calle zarogoza 52 41001 Sevilla See on map THE UNIVERSITY OF MILANO Italy EU contribution € 0,00 Address Via celoria 26 20133 Milano See on map UNIVERSITY OF ESSEX GB EU contribution € 0,00 Address Wivenhoe park john tabor laboratories CO4 3SQ Colchester See on map Links Website Opens in new window WESTFAELISCHE WILHELMS - UNIVERSITAET MUENSTER Germany EU contribution € 0,00 Address 3,corrensstrasse 3 48149 Muenster See on map
