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Exploiting genomics to engineer an environmentally friendly microorganism for bioremediation purposes

Objective

This project has been assembled to develop safer biologically contained strains for bioremediation purposes based on then on-pathogenic bacterium Pseudomonas putrid KT2440, which has been recognised as a safe strain for recombinant DNAtechnology by the NIH. For the first time, the availability of the entire genome of this bacterium will allow to establish the scientific basis for a rational design of environrnentally friendly genetically modified microbes (EF-GMM). Therefore, taking advantage of this novel information a fundamental aim of this proposal is to develop a large series of genetic and biochemical tools to target the incorporation of renal in stable sites of the host chromosome, in order to enhance the predictability of the ecological behaviour of the strain in polluted soils or bioreactors. Furthermore, these tools in combination with potent in silicon analyses will allow to establish the complete set of genes involved in the degradation ofbiogenic and xenophobic aromatic compounds that constitutes its derivative potential and is the master key to expand the bioremediation capabilities of this strain. The construction of DNA arrays will allow analysing and predicting how the introduction of foreign catabolic genes affects the global pattern of gene expression under different stress conditions. Finally, to verify commercialisation of the envisaged product ...

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Address
144,Velázquez 144
28071 Madrid
Spain

Participants (7)

BIOINFOBAK INSTITUTE
Poland
Address
Limanowskiego, 24/A
60 744 Poznan
HANNOVER MEDICAL SCHOOL
Germany
Address
Carl-neuberg-strasse 1
30625 Hannover
MINRAV INFRASTRUCTURES 1993 (LTD)
Israel
Address
Kiriat-moria
91291 Jerusalem
NEWBIOTECHNIC S.A.
Spain
Address
Calle Zarogoza 52
41001 Sevilla
THE UNIVERSITY OF MILANO
Italy
Address
Via Celoria 26
20133 Milano
UNIVERSITY OF ESSEX
United Kingdom
Address
Wivenhoe Park John Tabor Laboratories
CO4 3SQ Colchester
WESTFAELISCHE WILHELMS - UNIVERSITAET MUENSTER
Germany
Address
3,Corrensstrasse 3
48149 Muenster