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Exploiting synthetic SH2-scaffolded receptoire libraries to profile cancer cells and to interfere with cancer-related phenotypes

Objective

A new biotechnological process, exploiting scaffold synthetic repertoires will be developed to profile cancer cells by polemics, interfere with cancer phenotypes, rationally design drugs. The selected scaffold is the SH2 domain, an interaction surface, which binds to phosphotyrosine (pie)-containing peptides. By random autogenesis of five critical residues in the domain we have created library theoretically able to bind every containing-containing protein. This library will be used to
a) identify novel specificities of synthetic SH2 domains,
b) molecularly dissect the SH2: pY-peptide interaction,
c) purify novel pY-proteins,
d) interfere with phenotypes such as proliferation, differentiation and apoptosis,
e) profile containing patterns in cancer, f) develop peptide antagonists. The involvement of the SH2: pY interaction, in TK-mediated signalling, predicts application of this bioprocess to other pathological conditions, in addition to cancer, involving aproliferative/signaling component.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

ISTITUTO EUROPEO DI ONCOLOGIA SRL
Address
Via Ripamonti 435
20141 Milano
Italy

Participants (2)

CANCER RESEARCH TECHNOLOGY LIMITED
United Kingdom
Address
Sardinia House, Sardinia Street
WC2A 3NL London
FORSCHUNGSVERBUND BERLIN E.V.
Germany
Address
4,Robert-roessle-strasse 10
13125 Berlin