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Content archived on 2024-06-17

Upgrading lentiviral vectors for therapeutic gene delivery to resting cells still refractory to gene transfer

Objective

The candidate will extend her skills in handling and manipulating retroviral vectors to the more complexes subgroup of lent viruses, which prove to become a promising tool for gene therapy applications. She will receive training in the identification and culture of haematopoietic stem cells as also in analysis of these cells for their tot potency (in vitro essays and repopulation of NOD/SCID mouse) by FACS analysis. She will gain detailed knowledge on genetic manipulation of envelope lipoprotein and virus pseudotyping. A long-term goal of the host lab is to find solutions to allow in vivo gene delivery via the inoculation of integrative vectors derived from retroviruses. The host has the expertise to engineer upgraded retroviral vectors derived from marine leukaemia viruses, and found solutions to enhance their bio safety, capacity to specifically recognize target cells, improve vector titters and allow stability in human serum. More recently, the host lab developed lent viral vectors derived from primate lent viruses, which allow transudation of some non-proliferating cells. Here we are looking to make a further step toward the generation of vectors suitable for in vivo gene delivery by overcoming the problems that still limit gene transfer to some important gene therapy targets. It is expected that numerous applications in gene therapy will be possible after....

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Topic(s)

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Call for proposal

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Funding Scheme

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RGI - Research grants (individual fellowships)

Coordinator

ECOLE NORMALE SUPERIEURE DE LYON
EU contribution
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Allée d'Italie 46
69364 LYON
France

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Total cost

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