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Engineering yeast for efficient expression of heterologous membrane transporters

Objective

Expression of human proteins in a readily usable heterologous system is a powerful mean to study their function and structure and to discover novel targets and therapeutic routes directly associated with the causes of disease. Common yeast is ideal to adapt this technology to the human genome scale. Yet many laboratories have the same fundamental problem - the failure of many membrane proteins (the main target of pharmaceutical products) to be functionally expressed in yeast due to incorrect delivery or maintenance at the plasma membrane. The EFFEXPORT project aims to engineer yeast into a more reliable system for expression of heterologous membrane transporters. The novel yeast strains and associated strategies will be used to develop a new type of screening assay for identifying chemical compounds that act selectively on transporters of interest.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

UNIVERSITE LIBRE DE BRUXELLES
Address
Rue Des Pr. Jeener Et Brachet, 12
6041 Gosselies
Belgium

Participants (7)

INSTITUTE OF PHYSIOLOGY - CZECH ACADEMY OF SCIENCES
Czechia
Address
1083,Videnska 1083
142 20 Praha 4
JOHANN WOLFGANG GOETHE UNIVERSITAET FRANKFURT
Germany
Address
Seckenberg Anlage 31
60054 Frankfurt Am-main
LUDWIG INSTITUTE FOR CANCER RESEARCH - STOCKHOLM BRANCH
Sweden
Address
Karolinksa Institute
171 77 Stockholm
SYNOVO GMBH
Germany
Address
Paul-ehrilcih-str, 15
72076 Tuebingen
TEL AVIV UNIVERSITY
Israel
Address
Ramat-aviv
69978 Ramat - Aviv
THE ROYAL VETERINARY AND AGRICULTURAL UNIVERSITY
Denmark
Address
13,Buelowsvej 17
1870 Frederisksberg
THE UNIVERSITY OF TUEBINGEN
Germany
Address
Auf Der Morgenstelle 1
72076 Tuebingen