Engineering yeast for efficient expression of heterologous membrane transporters

Objective

Expression of human proteins in a readily usable heterologous system is a powerful mean to study their function and structure and to discover novel targets and therapeutic routes directly associated with the causes of disease. Common yeast is ideal to adapt this technology to the human genome scale. Yet many laboratories have the same fundamental problem - the failure of many membrane proteins (the main target of pharmaceutical products) to be functionally expressed in yeast due to incorrect delivery or maintenance at the plasma membrane. The EFFEXPORT project aims to engineer yeast into a more reliable system for expression of heterologous membrane transporters. The novel yeast strains and associated strategies will be used to develop a new type of screening assay for identifying chemical compounds that act selectively on transporters of interest.

Programme(s)

• FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002

Topic(s)

• 1.1.1.-3. - Key action The "Cell factory"

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

UNIVERSITE LIBRE DE BRUXELLES

Address

Rue Des Pr. Jeener Et Brachet, 12
6041 Gosselies
Belgium

Participants (7)

INSTITUTE OF PHYSIOLOGY - CZECH ACADEMY OF SCIENCES

Czechia

Address

1083,Videnska 1083
142 20 Praha 4

JOHANN WOLFGANG GOETHE UNIVERSITAET FRANKFURT

Germany

Address

Seckenberg Anlage 31
60054 Frankfurt Am-main

LUDWIG INSTITUTE FOR CANCER RESEARCH - STOCKHOLM BRANCH

Sweden

Address

Karolinksa Institute
171 77 Stockholm

SYNOVO GMBH

Germany

Address

Paul-ehrilcih-str, 15
72076 Tuebingen

TEL AVIV UNIVERSITY

Israel

Address

Ramat-aviv
69978 Ramat - Aviv

THE ROYAL VETERINARY AND AGRICULTURAL UNIVERSITY

Denmark

Address

13,Buelowsvej 17
1870 Frederisksberg

THE UNIVERSITY OF TUEBINGEN

Germany

Address

Auf Der Morgenstelle 1
72076 Tuebingen