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Content archived on 2024-05-24

Screening, cloning, statement and purification of thermostable enzymes for industrial applications

Objective

The cDNA library from thermopile bacteria will be constructed and screened for xylonite and protease activity. The genes encoding thermos table enzymes will be cloned into two expression systems. The best expression system will be used for expression and purification, and characterisation of each enzyme using chromatography.

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