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Content archived on 2024-06-17

Biosensors for in situ evaluation of bioavailability of pollutants based on transcriptional regulators a la carte.

Objective

This Project exploits the properties of prokaryotic transcriptional regulators of the XylR/HbpR family to design a la carte regulators that will be employed to determine the bioavailability of nitro-, broom- and chloroaromatic chemicals and biphenyl ethers. The core of this proposal is the application of high-throughput combinatory genetic technologies to the protein module that sets the specificity of the response to distinct aromatics. This will allow the generation and selection of regulator variants able to recognize and to trigger responses to a large variety of chemical species. The selected proteins will be employed in various biosensor schemes through the coupling of the cognate promoters to genetically formatted lox and gap reporters. This genetic standardisation will going parallel with matching instrumentation, hardware and software suited for measuring the optical output of the system and its utilisation in the field.

Fields of science (EuroSciVoc)

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Topic(s)

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Call for proposal

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Funding Scheme

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CSC - Cost-sharing contracts

Coordinator

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
EU contribution
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Address
1,Profesor Albareda 1
18008 GRANADA
Spain

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Total cost

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Participants (4)

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