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Content archived on 2024-06-17

Integrated strategy for efficient therapeutic retrovirus production based on modular cell lines

Objective

To increase successful applications of retrovirus delivery in gene therapy this demonstration project integrates and validates novel but robust technologies:
i) Specific genetically stable and safer human diploid cells yield higher virus titers than established, transformed cell lines;
ii) Modular master producer cell lines permit predictable expression properties of the therapeutic construct and envelope genes, inserted precisely at defined, optimal loci, conserving expression levels, physiological, genetic stability and safety advantages of the master cells;
iii)Medium, cultivation, purification and storage improvements result in maximal cell and tissue infectivity;
iv) Safety and efficacy of cells and retroviral vectors will be thoroughly tested and certified, including examination in solid tissue organ systems;
v) A final cGMP run will validate and complete the integrated strategy to produce clinically applicable material for trials against dystrophic epidermolysis bullosa.

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

DEM - Demonstration contracts

Coordinator

INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLOGICA
EU contribution
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Address
Quinta do Marques
2780-901 OEIRAS
Portugal

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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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Participants (4)

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