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Soil metagenomics to identify novel mechanisms of antagonism and antifungal activity for the improved control of phytopathogens

Objective

This project will explore & exploit the anti-phytopathogen diversity present in selected European suppressive soils representing a wide geographical spread. The untapped functional potential present in the unculturable part of the microbiota of these soils will be accessed using metagenomic cloning of soil DNA. Moreover, as a baseline, antagonistic organisms will also be isolated. Libraries constructed on the basis of the total and the active cell fractions, as well as the isolates, will be screened for anti-phytopathogen function. Selected clones will be as said with respect to the nature of the antagonistic compounds and production loci, involving sequencing. Potential producer strains will be optimised as to gene expression, growth and stability. Based on the gene sequences obtained, molecular tools will be developed and used to monitor soil suppressiveness. The impact (biosafety) of potential applications will be assessed.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

PLANT RESEARCH INTERNATIONAL B.V.
Address
Droevendaalsesteeg 1
Wageningen
Netherlands

Participants (7)

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
France
Address
43,Boulevard Du 11 Novembre 1918 , Bat 741
69622 Villeurbanne
FEDERAL BIOLOGICAL RESEARCH CENTRE FOR AGRICULTURE AND FORESTRY (BBA)
Germany
Address
Messeweg 11/12
38104 Braunschweig
LIBRAGEN S.A.
France
Address
Impasse Rtgaud 7
69300 Caluire Et Cuire
NATURAL ENVIRONMENT RESEARCH COUNCIL
United Kingdom
Address
North Star Avenue, Polaris House
SN2 1EU Swindon
NETHERLANDS RESEARCH SCHOOL FOR ASTRONOMY - LEGALLY REPRESENTED BY UNIVERSITY OF GRONINGEN
Netherlands
Address
Kerklaan 30
9751 NN Haren Gn
THE SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES
Sweden
Address
10,Arrheniusplan 2C
750 07 Uppsala
UNIVERSITE CLAUDE BERNARD LYON 1
France
Address
43 Boulevard Du 11 Novembre 1918 , Bat 741
69622 Villeurbanne