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Novel surfaces for switchable nucleic acids adsorption/ desorption for analytical assays

Objective

This proposal aims to provide platform technology for the rapid extraction and purification of genetic material (DNA and RNA) from any kind of sample (medical and environmental). End user applications cover all fields in which the analysis of genetic material is important, involving several key actions in various thematic programmes. The technology to be developed is based on novel organic dendrite functionalised surfaces switch able from an adsorbing state during the extraction phase to a desorbing state during the elution phase. The design of the dendrites is directed by rigorous chemical-chemical analysis of data on molecular-level interactions, mechanisms and relevant parameters obtained via in site evanescent wave monitoring of the functionalised surfaces during NA binding. Surfaces maximizing recovery rates will be incorporated in high-throughput automated instrumentation for NA recovery.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

NATIONAL RESEARCH COUNCIL OF ITALY
Address
Piazzale Aldo Moro 7
00185 Roma
Italy

Participants (4)

COLLEGIUM BASILEA (INSTITUTE OF ADVANCED STUDY)
Switzerland
Address
Hochstrasse, 51
4053 Basel
MICROVACUUM LTD.
Hungary
Address
10,Kerekgyarto U. 10
1147 Budapest
ROCHE INSTRUMENT CENTER AG
Switzerland
Address
Forrenstrasse
6343 Rotkreuz
UNIVERSITY OF BRIGHTON
United Kingdom
Address
Lewes Road, Mithras House
BN2 4GJ Brighton