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Content archived on 2024-05-24

Exploiting the secretion machinery of pseudomonads for the nanotechnological production of pharmaceuticals

Objective

The use of enzymes as biocatalysts is a rapidly developing field of immense academic and industrial interest. Several biocatalysts as lipases and penicillinacylases proved to be optimally suited for the Nan technological production of therapeutic compounds including enantiopure pharmaceuticals. At present, however, these biocatalysts cannot be produced in sufficient amounts and at a reasonable price because traditional production hosts like Bacillus and Aspergillums do not support their correct folding and secretion. This proposal aims at providing absolution to this problem by construction of a Gram-negative secretion host strain with its perils engineered into a compartment optimised for the assembly and folding of complex biocatalysts. The barrier in the secretion pathway imposed byte bacterial outer membrane will be overcome by relaxing the selectivity and specificity of the secretion machinery using Nan biotechnological strategies.

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Call for proposal

Data not available

Coordinator

UTRECHT UNIVERSITY
EU contribution
No data
Address
Padualaan 8
3584 CA UTRECHT
Netherlands

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Total cost
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Participants (7)