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Developing a genomic toolbox for exploring and exploiting bacterial biodiversity

Objective

Array based tools for the characterization and exploitation of all aspects of bacterial diversity will be developed: micro arrays using oligonucleotides and PCRproducts, as well as macro-arrays using PCR products. The technologies will be compared and evaluated. Work will focus on Sinorhizobium, bacteria capable of Unification and formation of root-nodules on leguminous plants, but the methods developed will be applicable to other bacterial groups and other genes /biological functions of interest. Initially a total S. maillot genome microarraywill be used to study the diversity of housekeeping and accessory genes. We will develop custom arrays for: species identification (the key to other data on an organism), screening for stress-tolerance genes (drought and salt stress are important factors affecting the efficiency of inoculants Sinorhizobium strains) and screening for genes involved in rhizobium-legume symbiosis.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

GENT UNIVERSITY
Address
Sint Pietersnieuwstraat 25
9000 Gent
Belgium

Participants (9)

ELOMESTARI OY
Finland
Address
Huttulantie 1, Partala
51900 Juva
INSTITUT DE RECHERCHE POUR LE DEVELOPPEMENT
France
Address
Rue La Fayette 213
Paris
LINKOEPING UNIVERSITY
Sweden
Address
Hus Origo Campus Valla
581 83 Linkoeping
NITRAGIN, INC
United States
Address
West Custer Avenue 3101
53209 Milwaukee,wisconsin
RESEARCH INSTITUTE FOR AGRICULTURAL MICROBIOLOGY
Russia
Address
Podbelsky Shosse 3
189620 Pushkin
UNIVERSITE D'ORAN ES-SENIA
Algeria
Address
Es-senia
31000 Oran
UNIVERSITY OF BIELEFELD
Germany
Address
Universitätsstraße 25
33501 Bielefeld
UNIVERSITY OF HELSINKI
Finland
Address
Yliopistonkatu 4
00014 Helsinki
University of York
United Kingdom
Address
Heslington
York