Array based tools for the characterization and exploitation of all aspects of bacterial diversity will be developed: micro arrays using oligonucleotides and PCRproducts, as well as macro-arrays using PCR products. The technologies will be compared and evaluated. Work will focus on Sinorhizobium, bacteria capable of Unification and formation of root-nodules on leguminous plants, but the methods developed will be applicable to other bacterial groups and other genes /biological functions of interest. Initially a total S. maillot genome microarraywill be used to study the diversity of housekeeping and accessory genes. We will develop custom arrays for: species identification (the key to other data on an organism), screening for stress-tolerance genes (drought and salt stress are important factors affecting the efficiency of inoculants Sinorhizobium strains) and screening for genes involved in rhizobium-legume symbiosis.
Funding SchemeCSC - Cost-sharing contracts
581 83 Linkoeping