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Development and validation of a dna-chip technology for the assessment of the bacteriological quality of bathing and drinking water

Objective

Pathogenic bacteria are a major health concern in drinking and bathing waters. Present day assessments of bacterial contaminations rely on culture methods, i.e. Plate counts of oviform bacteria, despite low reliability for detection of bacteria and their pathogen city. It is our objective to develop) molecular methods to identify and quantify pathogenic bacteria directly in any given water sample and to assess their state of activity. We will combine quantitative PCR and DNA-chip technology to retrieve specific gene sequences and assess their amounts in water samples. 16S renal sequences serve to identify species, mina of tuff genes and 16S reran indicate bacterial activity, pathogen city factors assess infectious risks. The applicability will be tested in relevant aquatic environments of the EU. The final objective is to create a novel technology for the EU-wide detection of waterborne pathogenic bacteria and to validate its mass application.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

GBF - NATIONAL CENTRE FOR BIOTECHNOLOGY
Address
Mascheroder Weg 1
38124 Braunschweig
Germany

Participants (4)

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
France
Address
Bètiment Jean Maetz, Chemin Du Lazaret 284, La Dar
06238 Villefranche-sur-mer
GENOLIFE S.A.
France
Address
Biopole Clermont Limagne
63360 Saint Beauzire
HYDRA INSTITUTE BUERO - DR KASIMIR KEG
Austria
Address
Himmelstrasse 42
1190 Wien
UNIVERSITE DE NICE - SOPHIA ANTIPOLIS
France
Address
Bètiment Jean Maetz, Chemin Du Lazaret 284, La Dar
06238 Villefranche-sur-mer