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Content archived on 2024-05-24

Detoxification of reactive oxygen species: molecular strategies

Objective

The first aim is to determine the mechanism whereby H202 acts as a signal inducing local and systemic glutathione accumulation in the C3 crop species, barley. The second aim is to identify and characterise the intracellular redo sensors and responsive-responsive transcription factors involved in sensing and relaying H202-induced stress signals to the nucleus and other responsive-responsive genes. A complete understanding of the molecular and metabolic factors involved in responsive signal transudation is fundamental to crop improvement but is, at present, lacking. Active oxygen species (AOS) such as H202 have been shown to mediate intra- and intercellular communication following exposure to pathogens and to environmental stresses. H202 is a key component in the hypersensitive response and the systemic acquired resistance (SAR) that follows attack by virulent pathogens. Details of the transudation sequence(s) through which local infection leads to SAR remain elusive, although both local and systemic increases in salicylic acid are key features. The first approach in this research programme involves barley mutants lacking a specific 53kDa catalyse. These plants provide an ideal system to explore mediated-mediated signal tradition. The second approach aims to identify signal transuding elements that respond to oxidative stress. Key to this approach is a deletion mutant in a transcription factor, YAP1, in S. cerevisiae. It is involved in the expression of oxidative stress-responsive genes.

Fields of science (EuroSciVoc)

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Topic(s)

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Call for proposal

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Funding Scheme

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RGI - Research grants (individual fellowships)

Coordinator

ROTHAMSTED RESEARCH LIMITED
EU contribution
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Address
West Common
AL5 2JQ HARPENDEN
United Kingdom

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Total cost

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