This project will investigate the feasibility of producing a diagnostic chip for the simultaneous detection of all the plant pests/pathogens present in the EU Plant Health Directive (77/93/EEC), using quarantine potato pests as a model system. The project will establish methods for detection of quarantine pathogens in plant tissue using gene chip technology. Up to 30000 DNA probes (sequences from each of the organisms to be tested in one assay) can be arrayed onto a microscope slide - the "gene chip". The chip can be exposed to fluorescent-labelled nucleic acid from the sample and fed into a micro-array reader to reveal if any of the pathogens were present. The project covers the whole process from preparing target genes, generating gene chips, preparing samples, hybridisation of samples, analysis of results and finally through to quarantine diagnosticians evaluating the technology.
Funding SchemeCSC - Cost-sharing contracts
46113 Moncada (Valencia)
Y010 5DD York