Testing the mitochondrial theory of ageing

Objective

The mitochondria theory of ageing proposes that the accumulation of somatic mutations in mitochondria DNA during life results in a progressive decline in energetic function and eventual cellular and organism senescence. We propose to test this idea directly, by engineering and expressing a version of the mitochondria DNA polymerise POLG that lacks proofreading exonuclease activity, and hence results in the rapid accumulation of medina mutations. This medina matador will be created and tested in parallel in four model systems: yeast, the mouse, cultured human cells and the fruit fly Drosophilae. The phenotypic consequences of an enhanced rate of accumulation of medina mutations will be investigated via studies of cellular bioenergetics function, ageing-related physiological decline and tissue degeneration, alterations in the pattern of gene expression, and overall lifespan.

Programme(s)

• FP5-LIFE QUALITY - Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002

Topic(s)

• 1.1.1.-6. - Key action The Ageing Population and Disabilities

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

UNIVERSITY OF TAMPERE

Address

6,Lenkkeilijänkatu 6
33014 Tampere
Finland

Participants (4)

ASTRA ZENECA AB

Sweden

Address

Soedertaelje
15185 Jaerna

KAROLINSKA INSTITUTE

Sweden

Address

Karolinska Hospital
171 76 Stockholm

UNIVERSIDAD AUTONOMA DE MADRID

Spain

Address

Arzobispo Morcillo N2
28029 Madrid

UNIVERSITE CATHOLIQUE DE LOUVAIN

Belgium

Address

Place Croix Du Sud 2/19
1348 Louvain-la-neuve