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Content archived on 2024-05-27

Analysis of gene expression in differentiating neurons using a cdna microarray approach.

Objective

The subject of this study is mRNA expression profiling of differentiating murine hippocampal neurons. A cDNA microarray approach will be used to simultaneously quantify expression levels of about seven thousand of mRNAs at morphologically and functionally defined stages of neuronal differentiation. A culture system will be used in which neurons undergo differentiation, in a well-coordinated manner, to a fully polarised pyramidal phenotype with functional synapses. The objectives of this study are:
1. quantitative analysis of expression of a large mRNA set at defined stages of neuronal differentiation
2. Identification of genes that are regulated in the differentiation process
3. Cluster analysis of genes according to their expression patterns. The results of this project will form a basis for future work; on regulatory mechanisms underlying coordinated expression of genes in differentiating neurons; and on functional relationships of protein products of the developmentally regulated genes.

Fields of science (EuroSciVoc)

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Topic(s)

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Call for proposal

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

RGI - Research grants (individual fellowships)

Coordinator

FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGY VZW
EU contribution
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Address
498BUS1,Herestraat 49 KUL / Campus Gasthuisberg
3000 LOUVAIN / LEUVEN
Belgium

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Total cost

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