PROGESTERONE IN HUMAN SERUM

Objective

Progesterone is a steroid hormone derived from cholesterol. In adult women, it is produced in the ovaries. An important reason for determining progesterone in serum is the assessment of female infertility.

The project includes the development of an isotope dilution - mass spectrometry (ID-MS) method for the determination of progesterone in human serum. Two serum pools will be prepared from blood collected from selected populations of donors. Each batch is to contain only endogenous progesterone.

RESULTS

The project has been completed in 1989. Six laboratories contributed to the establishment of the certified value for the progesterone concentration in each of the two serum pools. Four intercomparison exercises were necessary to set up an ID-MS procedure in each participating laboratory. The between laboratory CV was initially of about 6 %. The certified concentrations of progesterone in the serum reference materials are:
CRM 347 (10.13 +/- 0.21) nmol/l
CRM 348 (40.3 +/- 1.0) nmol/l
Details on the certification exercise are published in report EUR 12282 (1989).

Programme(s)

• FP2-BCR 4 - Research and development programme (EEC) in the field of applied metrology and chemical analysis (Community Bureau of Reference - BCR), 1988-1992

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Academisch Ziekenhuis

Address

Groningen
Netherlands

Participants (6)

Centro di Spettrometria di Massa

Italy

Address

501 Firenze

Fondation de Recherche en Hormonologie

France

Address

Fresnes

National Institute for Biological Standards and Control (NIBSC)

United Kingdom

Address

Blanche Lane South Mimms
EN6 3QG Potters Bar

Rheinische Friedrich-Wilhelms-Universität Bonn

Germany

Address

Sigmund-freud-straße 25
53127 Bonn

Rijksuniversiteit Gent

Belgium

Tenovus Institute for Cancer Research

United Kingdom

Address

Cardiff