Objective
Progesterone is a steroid hormone derived from cholesterol. In adult women, it is produced in the ovaries. An important reason for determining progesterone in serum is the assessment of female infertility.
The project includes the development of an isotope dilution - mass spectrometry (ID-MS) method for the determination of progesterone in human serum. Two serum pools will be prepared from blood collected from selected populations of donors. Each batch is to contain only endogenous progesterone.
RESULTS
The project has been completed in 1989. Six laboratories contributed to the establishment of the certified value for the progesterone concentration in each of the two serum pools. Four intercomparison exercises were necessary to set up an ID-MS procedure in each participating laboratory. The between laboratory CV was initially of about 6 %. The certified concentrations of progesterone in the serum reference materials are:
CRM 347 (10.13 +/- 0.21) nmol/l
CRM 348 (40.3 +/- 1.0) nmol/l
Details on the certification exercise are published in report EUR 12282 (1989).
Fields of science
Topic(s)
Data not availableCall for proposal
Data not availableFunding Scheme
CSC - Cost-sharing contractsCoordinator
Groningen
Netherlands