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PROGESTERONE IN HUMAN SERUM

Objective



Progesterone is a steroid hormone derived from cholesterol. In adult women, it is produced in the ovaries. An important reason for determining progesterone in serum is the assessment of female infertility.

The project includes the development of an isotope dilution - mass spectrometry (ID-MS) method for the determination of progesterone in human serum. Two serum pools will be prepared from blood collected from selected populations of donors. Each batch is to contain only endogenous progesterone.

RESULTS

The project has been completed in 1989. Six laboratories contributed to the establishment of the certified value for the progesterone concentration in each of the two serum pools. Four intercomparison exercises were necessary to set up an ID-MS procedure in each participating laboratory. The between laboratory CV was initially of about 6 %. The certified concentrations of progesterone in the serum reference materials are:
CRM 347 (10.13 +/- 0.21) nmol/l
CRM 348 (40.3 +/- 1.0) nmol/l
Details on the certification exercise are published in report EUR 12282 (1989).

Coordinator

Academisch Ziekenhuis
Address

Groningen
Netherlands

Participants (6)

Centro di Spettrometria di Massa
Italy
Address

501 Firenze
Fondation de Recherche en Hormonologie
France
Address

Fresnes
National Institute for Biological Standards and Control (NIBSC)
United Kingdom
Address
Blanche Lane South Mimms
EN6 3QG Potters Bar
Rheinische Friedrich-Wilhelms-Universität Bonn
Germany
Address
Sigmund-freud-straße 25
53127 Bonn
Rijksuniversiteit Gent
Belgium
Tenovus Institute for Cancer Research
United Kingdom
Address

Cardiff