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DIARRHEIC SHELLFISH POISONING (DSP)

Objective



DSP toxins are produced by marine algae and are accumulated by shellfish, particularly by mussels. More than five different DSP toxins have been identified so far, but two of them are predominant in Europe (okadaic acid and DTX-1). The consumption of DSP contaminated mussels can cause gastrointestinal disorder. DSP toxins are also considered to be potent tumour promoters. Contamination of shellfish by DSP occurs regularly in Europe and most Community Countries operate DSP monitoring schemes on imports of shellfish and at production sites. The European Community (DG VI - Agricultural Services) is preparing a Directive (COM (89) 645 final) which will establish limits of DSP toxins in shellfish. The method for DSP determination is normally the non-specific mouse/rat bioassay and, to a lesser extent, chemical analysis by HPLC. Development of chemical methods is being hampered by the interference of organic acids with the determination of DSP toxins and by the lack of pure toxins.
The aim is to provide samples of individual toxins, to develop a candidate official method and to raise the reliability of the determination by European laboratories.

STATUS

Significant quantities of okadaic acid have been produced from cultures of algae. Chromatographic methods for routine monitoring and absolute identification of DSP toxins are being developed. Solutions of okadaic acid, contaminated mussels and mussel extracts for intercomparisons are being prepared. An intercomparison involving some 20 laboratories to assess performance of HPLC methods will be completed in 1992.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

RIKILT
Address

6700 AE Wageningen
Netherlands

Participants (16)

CNEVA
France
Address
43 Rue De Dantzig
75015 Paris
Chemische Landesuntersuchungsanstalt Freiburg
Germany
Address
Bissierstraße 5
79114 Freiburg
Dept. of the Marine Fisheries Research Center
Ireland
Address
Abbotstown Castleknock
15 Dublin
Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)
France
Address
Station De Nantes Rue De L'ile D'yeu
44037 Nantes
Istituto Superiore della Sanità
Italy
Address
Viale Regina Elena 299
00161 Roma
Landesuntersuchungsamt für das Gesundheitswesen Sudbayern
Germany
Address
Veterinärstraße 2
85764 Oberschleissheim
Ministerio de Sanidad y Consumo
Spain
Address
Carretera Majadahonda - Pozuelo, Km 2,2
28220 Majadahonda (Madrid)
Ministerio de Sanidad, U.A.
Spain
Address
1552,Apartado De Correos
36280 Vigo
Ministry of Agriculture, Fisheries and Food (MAFF)
United Kingdom
Address
135 Abbey Road
AB9 8DG Aberdeen
Rijksinstituut voor Volksgezondheid en Milieuhygiene (RIVM)
Netherlands
Address

3720 BA Bilthoven
Robert-von-Ostertag-Institut
Germany
Address
Diedersdorfer Weg 1
12277 Berlin
Royal Veterinary and Agricultural University
Denmark
Address
12,Bülowsvej
1870 Copenhagen V
Staatliches Veterinäruntersuchungsamt für Fische und Fischware Cuxhaven
Germany
Address
Schleusenstraße
27472 Cuxhaven
University of Göteborg
Sweden
Address
10,Guldhedsgatan
41346 Göteborg
Universität Jena
Germany
Address
Dornburgerstraße 25-29
07743 Jena
Université Libre de Bruxelles
Belgium
Address
50,Avenue F. Roosevelt
1050 Bruxelles