Objective
DSP toxins are produced by marine algae and are accumulated by shellfish, particularly by mussels. More than five different DSP toxins have been identified so far, but two of them are predominant in Europe (okadaic acid and DTX-1). The consumption of DSP contaminated mussels can cause gastrointestinal disorder. DSP toxins are also considered to be potent tumour promoters. Contamination of shellfish by DSP occurs regularly in Europe and most Community Countries operate DSP monitoring schemes on imports of shellfish and at production sites. The European Community (DG VI - Agricultural Services) is preparing a Directive (COM (89) 645 final) which will establish limits of DSP toxins in shellfish. The method for DSP determination is normally the non-specific mouse/rat bioassay and, to a lesser extent, chemical analysis by HPLC. Development of chemical methods is being hampered by the interference of organic acids with the determination of DSP toxins and by the lack of pure toxins.
The aim is to provide samples of individual toxins, to develop a candidate official method and to raise the reliability of the determination by European laboratories.
STATUS
Significant quantities of okadaic acid have been produced from cultures of algae. Chromatographic methods for routine monitoring and absolute identification of DSP toxins are being developed. Solutions of okadaic acid, contaminated mussels and mussel extracts for intercomparisons are being prepared. An intercomparison involving some 20 laboratories to assess performance of HPLC methods will be completed in 1992.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences organic chemistry organic acids
- natural sciences biological sciences microbiology phycology
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Coordinator
6700 AE WAGENINGEN
Netherlands
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Participants (16)
75015 Paris
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79114 FREIBURG
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15 DUBLIN
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44037 Nantes
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00161 Roma
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85764 OBERSCHLEISSHEIM
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28220 MAJADAHONDA (MADRID)
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36280 VIGO
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AB9 8DG Aberdeen
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3720 BA BILTHOVEN
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12277 Berlin
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1870 COPENHAGEN V
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27472 CUXHAVEN
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41346 GÖTEBORG
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07743 JENA
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1050 BRUXELLES
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