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Short-term mutagenicity testing

Objective



Assessment of the toxicity of new chemicals is required by the EEC Directive 79/831, 6th amendment, and includes the determination of risks which could lead to cancer or to congenital malformation caused by genetic mutation.
The first level of screening consists in determining the genotoxicity of chemicals through a series of assays on micro-organisms and mammalian cells. Unfortunately, the results of these assays greatly depend upon how the methods are applied and upon the lines of bacterial strains used in performing the tests.
The objectives of the project are to prepare reference materials and to certify the mutagenic activity with a well defined, optimized procedure.

STATUS

A first intercomparison on mutagenicity testing has been carried out without metabolic activation (-S9) and showed up serious difficulties in obtaining results with a good agreement. In order to prepare the 2nd intercomparison with metabolic activation (+S9) a preliminary study (with three labs) was started so as to investigate:
1) some of the most important parameters of variability of this test (-S9) like
- type of solvents, agar, glucose sterilization, inducer
- concentration of mutagen;
2) the appropriate S9-mix
- rat strain
- concentration of S9.
Part 1 of this study has been carried out. Results will be available by the end of May 1992.
Part 2 will be terminated by end of summer 1992. The analytical protocol for the 2nd intercomparison will be adopted according to the results of the preliminary study.
Two highly purified indirect mutagens (benzo(a)pyrene and 2-aminoanthracene) have been prepared as candidate reference materials. HPLC, GC-FID and GC-MS analysis indicated 99.2% purity of benzo(a)pyrene and 99.9% purity for 2-aminoanthracene. Homogeneity and stability studies (12 months) have been successfully completed.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Biochemisches Institut für Umweltcarcinogene
Address
Sieker Landstraße 19
22926 Ahrensburg
Germany

Participants (17)

British Industrial Biological Research Association (BIBRA)
United Kingdom
Address
Woodmansterne Road
SM5 4DS Carshalton
Central Public Health Laboratory
United Kingdom
Address
61 Colindale Avenue
NW9 5HT London
Central Toxicology Laboratory
United Kingdom
Address

Chester
INSTITUT PASTEUR
France
Address
Rue Du Docteur Roux 25
75724 Paris
INSTITUT PASTEUR DE LILLE
France
Address
Rue Du Professeur Calmette 1
59019 Lille
Institut d'Hygiene et d'Epidemiologie
Belgium
Address

Bruxelles
Inveresk Research International Ltd
United Kingdom
Address

EH33 2NE Tranent
Microtest Research Ltd
United Kingdom
Address
Heslington
YO1 5DD York
National Research Center 'Democritus'
Greece
Address

Paraskevi Attikis
RCC Notox
Netherlands
Address

'S-hertogenbosch
SCK/CEN
Belgium
Address

Mol
Scantox A/S
Denmark
Address

Skensveld
Universitate Autonoma de Barcelona
Spain
Address

Bellaterra
Universite Catholique de Louvain
Belgium
Address

Bruxelles
Università degli Studi di Genova
Italy
Address
Viale Benedetto Xv 1-3
16126 Genova
Università degli Studi di Pisa
Italy
Address

56100 Pisa
Universität-Gesamthochschule Essen
Germany
Address
Hufelandstraße 55
45147 Essen