Objective Lactate dehydrogenase (LD) activity determinations in serum are being used, since years, for diagnosing many diseases involving heart, erythrocytes, liver and for detecting tumours. The serum contains in variable proportions five LD isoenzymes, formed from monomeric polypeptides B and A. The B4 isoenzyme - LD1 - is more abundant in the cells of heart muscle, kidney, brain and in erythrocytes. The LD1 determination in serum is particularly useful in the biochemical diagnosis of myocardial infarction. The objective of the project is to work both on a reliable method for the determination of the catalytic concentration of LD and on a reference enzyme preparation enabling the transfer of that method. The LD1 isoenzyme will be purified from human erythrocytes. STATUS A preliminary study has shown that a stable LD1 could be prepared. A batch of about 1500 samples is available for certification. A first intercomparison with 11 laboratories has been performed in 1989 with the following results : between-laboratory CV = 13.9% and within-laboratory CV = 4.2% on average. The followed method was that recommended by the French Society for Clinical Biology (SFBC). After several points of the methodology have been assessed (e.g. linearity of the reaction and purity of the reagents), much improvement was recorded. 8 participants will be involved in the value assignment campaign (due in 1992) with the SFBC method. Certification is expected in 1993. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programme(s) FP2-BCR 4 - Research and development programme (EEC) in the field of applied metrology and chemical analysis (Community Bureau of Reference - BCR), 1988-1992 Topic(s) Data not available Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator Hospices Civils de Lyon Address Lyon France See on map EU contribution € 0,00 Participants (8) Sort alphabetically Sort by EU Contribution Expand all Collapse all Boehringer Mannheim GmbH Germany EU contribution € 0,00 Address Penzberg See on map Centre Hospitalier Universitaire Vaudois Switzerland EU contribution € 0,00 Address Lausanne See on map Centre du Médicament France EU contribution € 0,00 Address Nancy See on map Fondazione Centro San Romanello del Monte Tabor Italy EU contribution € 0,00 Address 201 Milano See on map Hôpital Necker - Enfants Malades France EU contribution € 0,00 Address 149 rue de sèvres 75743 Paris See on map National Institute for Biological Standards and Control (NIBSC) United Kingdom EU contribution € 0,00 Address Blanche lane south mimms EN6 3QG Potters bar See on map Universidad Autónoma de Barcelona Spain EU contribution € 0,00 Address Barcelona See on map Université de Strasbourg I (Université Louis Pasteur) France EU contribution € 0,00 Address 67070 Strasbourg See on map Links Website Opens in new window
