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LACTATE DEHYDROGENASE

Objective



Lactate dehydrogenase (LD) activity determinations in serum are being used, since years, for diagnosing many diseases involving heart, erythrocytes, liver and for detecting tumours. The serum contains in variable proportions five LD isoenzymes, formed from monomeric polypeptides B and A. The B4 isoenzyme - LD1 - is more abundant in the cells of heart muscle, kidney, brain and in erythrocytes. The LD1 determination in serum is particularly useful in the biochemical diagnosis of myocardial infarction.

The objective of the project is to work both on a reliable method for the determination of the catalytic concentration of LD and on a reference enzyme preparation enabling the transfer of that method. The LD1 isoenzyme will be purified from human erythrocytes.

STATUS

A preliminary study has shown that a stable LD1 could be prepared. A batch of about 1500 samples is available for certification. A first intercomparison with 11 laboratories has been performed in 1989 with the following results : between-laboratory CV = 13.9% and within-laboratory CV = 4.2% on average. The followed method was that recommended by the French Society for Clinical Biology (SFBC). After several points of the methodology have been assessed (e.g. linearity of the reaction and purity of the reagents), much improvement was recorded. 8 participants will be involved in the value assignment campaign (due in 1992) with the SFBC method. Certification is expected in 1993.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Hospices Civils de Lyon
Address

Lyon
France

Participants (8)

Boehringer Mannheim GmbH
Germany
Address

Penzberg
Centre Hospitalier Universitaire Vaudois
Switzerland
Address

Lausanne
Centre du Médicament
France
Address

Nancy
Fondazione Centro San Romanello del Monte Tabor
Italy
Address

201 Milano
Hôpital Necker - Enfants Malades
France
Address
149 Rue De Sèvres
75743 Paris
National Institute for Biological Standards and Control (NIBSC)
United Kingdom
Address
Blanche Lane South Mimms
EN6 3QG Potters Bar
Universidad Autónoma de Barcelona
Spain
Address

Barcelona
Université de Strasbourg I (Université Louis Pasteur)
France
Address

67070 Strasbourg