Skip to main content

PROSTATIC ACID PHOSPHATASE

Objective



Measurement of the enzyme prostatic acid phosphatase (PAP) in serum is an established procedure in monitoring metastatic carcinoma of the prostate and is also useful in confirming suspected early stage disease.

The project aims at preparing purified acid phosphatase form human prostate. The project also includes the investigation and subsequent development of an assay method of sufficient reliability and robustness for assigning a value to the PAP concentration in the enzyme material.

RESULTS

The purification of acid phosphatase from human prostatic tissue has been successful. A batch of 3000 samples in lyophilised form was prepared for certification. The method used was the one with 4-nitrophenyl phosphate as substrate and measurements were carried out at 37 C. 9 laboratories were involved and the following value and its uncertainty were proposed for certification:
CRM 410 (28.0 +/- 0.7) U/l.
The report describing the preparation and the certification exercise is being finalized for publication.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Royal Postgraduate Medical School
Address
150,Ducane Road
W12 ONN London
United Kingdom

Participants (9)

Boehringer Mannheim GmbH
Germany
Address

Penzberg
Centre Hospitalier Universitaire Vaudois
Switzerland
Address

Lausanne
Fondazione Centro San Romanello del Monte Tabor
Italy
Address

201 Milano
Hôpital Necker - Enfants Malades
France
Address
149 Rue De Sèvres
75743 Paris
Metaxas Memorial Cancer Hospital
Greece
Address

Piraeus
National Institute for Biological Standards and Control (NIBSC)
United Kingdom
Address
Blanche Lane South Mimms
EN6 3QG Potters Bar
Odense University Hospital
Denmark
Universidad Autonoma de Barcelona
Spain
University of Thessaloniki
Greece