Objective
Alpha-Foetoprotein (alpha-FP) is a fetal globulin synthetized by the embryonic liver. In human beings the transition form alpha-FP to albumin as the dominant serum protein begins normally at the thirteenth week of gestation. Elevated serum levels of alpha-FP in adults have been found in patients with primary liver cancer, gonadal tumours and carcinomas of other tissues.
The project will include the assessment of the suitability of alpha-FP purified from a hepatoma cell culture for calibration of serum alpha-FP assays. The project could lead to the preparation and certification of serum reference samples.
STATUS
A purified alpha-FP preparation form hepatoma cell line has been produced at the Sclavo Institute (Siena, I). The material as stated by the manufacturer, is >99 % pure. It is foreseen to use it as calibrator for the determination of the mass concentration of alpha-FP in serum reference materials. Further assessment of the identity and purity of the alpha-FP has been carried out while comparison of methods for measuring serum alpha-FP proceeds. Some 20 laboratories were involved in interlaboratory studies. A lot of 2500 ampoules of lyophilized purified alpha-FP is prepared. 9 laboratories will be involved in the value assignment campaign and certification is expected in 1993.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences clinical medicine oncology liver cancer
- natural sciences biological sciences biochemistry biomolecules proteins
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Coordinator
CF Cardiff
United Kingdom
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Participants (35)
79098 Freiburg
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69260 Charbonnières-les-Bains
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Gouda
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Penzberg
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Nancy
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Bruxelles
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Glostrup
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Glostrup
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Marnes La Coquette
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EH3 9YW Edinburgh
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Liège
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30625 HANNOVER
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65926 Frankfurt am Main
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Barcelona
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13009 Marseille
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Fleurus
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81377 München
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Nice
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37077 Göttingen
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23562 Lübeck
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Athens
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EN6 3QG Potters Bar
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Cambridge
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Gif-sur-Yvette
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37079 Göttingen
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53100 Siena
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Woking
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Salluggia
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Nijmegen
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20148 HAMBURG
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