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ALPHA-FOETOPROTEIN

Objective



Alpha-Foetoprotein (alpha-FP) is a fetal globulin synthetized by the embryonic liver. In human beings the transition form alpha-FP to albumin as the dominant serum protein begins normally at the thirteenth week of gestation. Elevated serum levels of alpha-FP in adults have been found in patients with primary liver cancer, gonadal tumours and carcinomas of other tissues.
The project will include the assessment of the suitability of alpha-FP purified from a hepatoma cell culture for calibration of serum alpha-FP assays. The project could lead to the preparation and certification of serum reference samples.

STATUS

A purified alpha-FP preparation form hepatoma cell line has been produced at the Sclavo Institute (Siena, I). The material as stated by the manufacturer, is >99 % pure. It is foreseen to use it as calibrator for the determination of the mass concentration of alpha-FP in serum reference materials. Further assessment of the identity and purity of the alpha-FP has been carried out while comparison of methods for measuring serum alpha-FP proceeds. Some 20 laboratories were involved in interlaboratory studies. A lot of 2500 ampoules of lyophilized purified alpha-FP is prepared. 9 laboratories will be involved in the value assignment campaign and certification is expected in 1993.

Coordinator

Amersham International plc
Address

CF Cardiff
United Kingdom

Participants (35)

Albert-Ludwigs-Universität Freiburg
Germany
Address
Werthmannsplatz
79098 Freiburg
BioMérieux SA
France
Address
Marcy L'etoile
69260 Charbonnières-les-bains
Bleuland Ziekenhuis
Netherlands
Address

Gouda
Boehringer Mannheim GmbH
Germany
Address

Penzberg
Centre du Médicament
France
Address

Nancy
Cliniques Universitaires St. Luc
Belgium
Address

Bruxelles
Copenhagen County Hospital
Denmark
Address

Glostrup
Dakopatts
Denmark
Address

Glostrup
Diagnostics Pasteur-Kallestad
France
Address

Marnes La Coquette
Edinburgh Royal Infirmary
United Kingdom
Address

EH3 9YW Edinburgh
Eurogentec S.A.
Belgium
Address

Liège
HANNOVER MEDICAL SCHOOL
Germany
Address
Carl-neuberg-strasse 1
30625 Hannover
Herlev Hospital
Denmark
Hoechst AG
Germany
Address
Brüningstraße
65926 Frankfurt Am Main
Hospital de la Sante Creu i San Pau
Spain
Address

Barcelona
INSTITUT GUSTAVE ROUSSY
France
Immunotech SA
France
Address
130 Avenue De Lattre De Tassigny
13009 Marseille
Institut des Radioéléments-Medgenix
Belgium
Address

Fleurus
Klinikum München-Großhadern
Germany
Address
Marchioninistraße 15
81377 München
Laboratoires d'Analyses Biologiques Spécialisées
France
Address

Nice
Max-Planck-Gesellschaft zur Förderung der Wissenschaften eV
Germany
Address
Am Faßberg 11
37077 Göttingen
Medizinische Universität Lübeck
Germany
Address
Ratzeburger Allee 160
23562 Lübeck
National Athletic Research Centre
Greece
Address

Athens
National Institute for Biological Standards and Control (NIBSC)
United Kingdom
Address
Blanche Lane South Mimms
EN6 3QG Potters Bar
Novo Biolabs Ltd.
United Kingdom
Address

Cambridge
Oris-Industrie
France
Address

Gif-sur-yvette
SERATEC Gesellschaft für Biotechnologie mbH
Germany
Address
Rudolf-winkel-straße 1A
37079 Göttingen
Sclavo Sa
Italy
Address

53100 Siena
Serono Diagnostics Ltd.
United Kingdom
Address

Woking
Sorin Biomedica SpA
Italy
Address

Salluggia
St. Radboud Ziekenhuis
Netherlands
Address

Nijmegen
Statens Serumsinstitut Copenhagen
Denmark
UNIVERSIDADE DE COIMBRA
Portugal
Address
Hospitais Da Universidade De Coimbra - Praceta Mota Pinto
UNIVERSITY OF HAMBURG
Germany
Address
Moorweidenstrasse, 18
20148 Hamburg
VRIJE UNIVERSITEIT AMSTERDAM
Netherlands