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17 BETA-OESTRADIOL IN HUMAN SERUM

Objective



Oestradiol is a C18-steroid and is found in both females and males. In females, throughout puberty, it stimulates growth of the sex organs and development of secondary sexual characteristics, while in adult females its main biological action is on the functioning of the reproductive tract. In the male the role of oestradiol is thought to be involved with testosterone in the regulation of gonadotrophin secretion. Oestradiol assay is the most widely used test for the assessment of oestrogen status in infants and adults. Oestradiol analysis is also essential for monitoring ovulation inducing therapies and hormone replacement after castration or in the post-menopausal period of life.
The project aims at the certification of 17beta-oestradiol concentrations in human serum materials, by method procedures using isotope dilution - mass spectrometry (ID-MS).

STATUS

The feasibility of preparation and conservation of human serum based samples was sucessfully demonstrated. The method development achieved allows to envisage a certification exercise in 1992.
The project is actually at the stage of collecting the required human sera. Certification is expected in 1993 - 1994.

Coordinator

Centro di Spettrometria di Massa
Address

501 Firenze
Italy

Participants (6)

Fondation de Recherche en Hormonologie
France
Address

Fresnes
Instand
Germany
Address

Düsseldorf
National Institute for Biological Standards and Control (NIBSC)
United Kingdom
Address
Blanche Lane South Mimms
EN6 3QG Potters Bar
Rheinische Friedrich-Wilhelms-Universität Bonn
Germany
Address
Sigmund-freud-straße 25
53127 Bonn
Rijksuniversiteit Gent
Belgium
Tenovus Institute for Cancer Research
United Kingdom
Address

Cardiff