Objective
Osteocalcin (bone-gla protein, BGP), a non-collagenous protein from bone matrix, was suggested to be a reliable non-invasive marker for prevention and diagnosis in many bone diseases such as osteoporosis. Indeed, osteoporosis is one of the most common metabolic bone diseases resulting in bone loss responsible for vertebral or femoral fractures in post-menopausal women or in women and men over 70 years. For example, in France, about 30.000 hip fractures per year were reported in osteoporotic people and about 1.000.000 women were carriers of vertebral crushes. In Sweden, the Netherlands and Great Britain, similar incidences were described.
The aim of the project is to employ the techniques of genetic engineering and cell biology to produce purified human osteocalcin. The cDNA coding for osteocalcin will be cloned and appropriate vectors will be tried to express the cDNA in eukaryotic cells. Additionally, the possibility of obtaining osteocalcin route from osteoblast cultures will be investigated.
STATUS
The project has started in 1990. Using polymerase chain reaction and specific oligonucleotides as primers, amplification of the human BGP gene sequences was successfully achieved. The human BGP gene was then cloned in the pBR 322 plasmid and ligated to an eukaryotic expression vector. Gene transfer experiments are in progress. At the completion of the project, a limited amount of purified material should be available for further studies.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences medical biotechnology genetic engineering gene therapy
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences cell biology
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
Nancy
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.