Skip to main content

DEVELOPMENT OF A COMMUNITY METHOD FOR THE DETERMINATION OF BETA-AGONISTS IN ANIMAL FEED

Objective


The methods needed to detect a wide range of beta agonists focus on immunoaffinity columns coupled to high performance liquid chromatography (HPLC) or gas liquid chromatography (GLC). An intercomparison of method involving 13 laboratories identified problems of extraction and clean up. An extraction and clean up study involving 4 laboratories was set up and should lead to a candidate modular Community method.
Beta-agonists are widely used in human medicine for the treatment of asthma and certain heart conditions. At higher concentrations, they also have the ability to increase the proportion of lean meat relative to fat in farm animal and to increase total weight with obvious financial benefit for the producers.

Although this practice is forbidden by Community legislation (Dir EEC/86/469) there are many examples of illegal use. The compounds are added to animal feed or drinking water and recently, a major feed company was prosecuted for incorporating the compounds in their products. Claims that beta-agonists are present in animal feed have recently resulted in one Member State banning imports from another. The Community is therefore in urgent need of a method to regulate and resolve such disputes and enforce legislation. The methods need to be capable of detection of a wide range of compounds and will probably focus on immuno-affinity columns coupled to HPLC or GLC.

STATUS

The first intercomparison of methods involving 13 laboratories identified problems of extraction and clean-up. An extraction and clean-up study involving 4 laboratories was started in 1991 and should lead to a candidate "modular" Community method. The proposed method will be tested in a final intercomparison in 1992.

Coordinator

Rijksontledingslaboratorium
Address
59,Braemkasteelstraat
9050 Gentbrugge
Belgium

Participants (18)

Agriparadigma Srl
Italy
Address
Via Faentina 224
48100 Ravenna
Boehringer-Ingelheim Vetmedica GmbH
Germany
Address

55218 Ingelheim
CSIC - Instituto de Fermentaciones Industriales
Spain
Address
3,Calle Juan De La Cierva
28006 Madrid
Dr. L. Willems-Instituut vzw
Belgium
Address
Universitaire Campus
3590 Diepenbeek
Institut d'Hygiène et d'Epidémiologie
Belgium
Address
14,Rue J. Wytsman
1050 Bruxelles
Institut für Zoo- und Wildtierforschung
Germany
Address
Alfred-kowalke-straße 17
10315 Berlin
Istituto Superiore della Sanità
Italy
Address
Viale Regina Elena 299
00161 Roma
Laboratoire Interrégional du DGCCRF
France
Address
26 Avenue Du Coëtlogon
35000 Rennes
Landeshauptstadt Stuttgart
Germany
Address
Staffelbergstraße 81
70184 Stuttgart
MAFF CENTRAL VETERINARY LABORATORY
United Kingdom
Address
New Haw
K115 3NB Weybridge
MAFF Food Science Laboratory
United Kingdom
Address
Norwich Research Park Colney
NR4 7UQ Norwich
Ministerio de Sanidad y Consumo - Instituto de Salud Carlos III
Spain
Address
Carretera Majadahonda - Pozuelo, Km 2,2
28220 Majadahonda (Madrid)
R J Heitzman
United Kingdom
Address
1 Churn Road Compton
RG16 0PP Newbury
RIKILT
Netherlands
Address

6700 AE Wageningen
Rijksinstituut voor Volksgezondheid en Milieuhygiene (R.I.V.M)
Netherlands
Address

3720 BA Bilthoven
Robert-von-Ostertag-Institut
Germany
Address
Diedersdorfer Weg 1
12277 Berlin
State Laboratory
Ireland
Address
Abbotstown - Castleknock
15 Dublin
École Nationale Vétérinaire de Nantes
France
Address
Route De Gachet
44087 Nantes