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Content archived on 2024-04-16

DETERMINATION OF AMINO ACIDS IN ANIMAL FEED Development of a Community Method and Reference Materials


The efficiency of animal feed, especially for intensive farming, depends heavily on the contents of several amino acids (methionine, threonine, cystine, lysine and tryptophan).
These compounds are commonly added to low grade protein waste materials to produce feed with sufficient nutritive value. Community legislation (Directive 77/101/EEC and 79/373/EEC) obliges declaration of certain of these compounds in feeds for specified animals and lays down the acceptable tolerances for the accuracy of the declaration. However, several Member States have indicated that they will refuse further development of this legislation until a Community method is adopted.
The objective is therefore to develop a Community method and reference feeds to assure its correct application.


After two intercomparisons, it has been shown that there were no detectable differences in the results of 14 laboratories even though the proposed candidate method (based on ion-exchange chromatography) allowed four variations (open and closed vessel, HBr and metabisulphite oxidation). The method will be recommended as a candidate Community method.
The preparation of reference materials (mixed pig feed, low fat fish meal, soya bean meal, skim milk powder and a calibration solution) has been completed and certification will be undertaken in 1992.


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