The current project is undertaken as a consequence of an intercomparison made under project no 341 which identified serious difficulties in the analysis of vitamins B1, B2 and B6.
The aim of this project is to improve the extraction and dephosphorylation procedures for these vitamins. The following will be investigated: choice of enzyme, pH, temperature, incubation time and sample:enzyme ratio. Further intercomparisons will involve the certification of these vitamins in reference materials prepared under project no 341 (vitamin enriched milk powder, flour, brussels sprouts) and Project 406 (lyophilized liver).
The first study designed to optimize the method is underway and will be completed in Spring 1992.
Funding SchemeCSC - Cost-sharing contracts
TW11 0LY Teddington
6700 AE Wageningen