Fine mapping of the leading strand replication origin of sea urchin mitochondrial deoxyribonucleic acid (mtDNA) has been achieved using riboprobe analysis. 2 developmentally regulated mitochondrial DNA binding proteins, each with multiple binding sites in the major replication pause region of sea urchin mtDNA have been characterised. Binding sites for DNA binding proteins around the leading strand replication origin of sea urchin mtDNA have been identified. 2 developmentally regulated mitochondrial RNA binding proteins, interacting with sense strand transcripts across an overlapping gene junction in sea urchin mtDNA have been detected and partially characterised. Preliminary characterisation of sea urchin mitochondrial ribonucleic acid (RNA) polymerase and its transcriptional activities in vitro has been achieved. Molecular probes for sea urchin ATP synthase genes have been created by PCR. The successful immunopurification of the antibody against sea urchin ATP synthase subunit alpha has been demonstrated.
Funding SchemeCSC - Cost-sharing contracts