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DEFINITION OF THE STRUCTURAL REQUIREMENTS AND THE THYROGLOBULIN MOLECULE FOR THYROID HORMONE FORMATION

Objective



Peptides corresponding to defined segments of rat thyroglobulin will be synthesized in E. coli. The in vitro synthesized polypeptides will be submitted to iodination under conditions favoring iodination and/or iodination plus coupling. The iodoamino acids distribution of the different fragments will be determined, the peptides containing thyroid hormones will be puridied by HPLC and sequenced to establish if hormone synthesis occurs at the same tyrosines used in vivo. Both structural and mutagenic analysis of such fragments should allow to deduce the minimal requirements for thyroid hormone biosynthesis.

Coordinator

EUROPEAN MOLECULAR BIOLOGY LABORATORY
Address
Meyerhofstrasse 1
Heidelberg
Germany

Participants (3)

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain
Address
Calle Serrano 117
Madrid
INSTITUTO NACIONAL DE LA SALUD
Spain
Address
Km9100,crta.de Colmenar Km 9
28034 Madrid
Università degli Studi di Udine
Italy
Address
Via Gervasutta 48
33100 Udine