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Content archived on 2024-04-16

Interaction entre un antigène viral et la réponse immunitaire humorale

Objective



We propose to define and structurally interpret the molecular bases of influenza virus haemagglutinin antigenic variations; these are observed under the selective pressure of the humoral immune response. Crystallographic studies of entities involved in this process (haemagglutinin mutants, Fab fragments of antibodies specific for haemagglutinin and complexes of these with the haemagglutinin) complemented by a study of their association kinetics will allow to set this problem in a well defined frame. The affinity of a monoclonal antibody for a viral mutant selected by growth in its presence will be compared to those of the same antibody for other mutants obtained by site specific mutagenesis and located in the antigenic site this body recognizes. These results should allow us to establish whether mechanisms other than affinity lowering are involved in the selection giving rise to antigenic mutants and to analyze them.

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Coordinator

Centre National de la Recherche Scientifique (CNRS)
EU contribution
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Address

91405 Orsay
France

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Participants (2)

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