Objective
The proposal aims at a concerted effort to explore the molecular mechanism of sarcoplasmic reticulum Ca2+-ATPase, a representative of the P type cation transporting ATPases. Ca2+-ATPase structure will be studied by the use of proteolytic dissection, immunolocalization, and chemical modification experiments to define the location of membrane buried and exposed segments of the polypeptide chain and Ca2+ transport sites. Molecular biology tools will be used to develop a yeast system to express relatively large amounts of Ca2+-ATPase mutants to pinpoint amino acid residues involved in Ca2+-binding. Functionally oriented studies will include examination of Ca2+-binding and -release by rapid kinetic experiments. In addition domain organization and proximity-relationships of strategic sites (ATP, phosphorylation, and Ca2+ binding) will be studied by fluorescence experiments, reaction with site-specific antibodies, and differential scanning calorimetry. The results obtained will be combined in an attempt to produce a functionally relevant structural model for Ca2+-
ATPase.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences chemical sciences analytical chemistry calorimetry
- natural sciences chemical sciences organic chemistry amines
- natural sciences biological sciences molecular biology
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Programme(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
8000 Århus C
Denmark
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