We are interested in a class of mobile elements known as LINES which, although they are not structurally related to retroviral-like elements, transpose by reverse transcription of an RNA intermediate.
We want to study the molecular mechanism of the transposition process and its control by using two LINE-like elements of Drosophila melanogaster. They are the I factors that are involved in the determination of the I-R system of hybrid dysgegenesis, and the F elements.
We shall try to determine the coding sequences of the I factor that are involved in the control of its activity. We shall also identify the Drosophila genes coding for products interacting with I element sequences. A fraction of these genes are expected to modify, when mutated, the phenotype of mutant alleles due to I element insertions. We shall study the properties of the F. element when introduced into another species and compare the structure of its promoter to that of the I factor.
Funding SchemeCSC - Cost-sharing contracts
EH9 3Jr Edinburgh