Objective
The NAM1 gene in Saccharomyces cerevisiae was isolated as a multicopy suppressor of mitochondrial mutations affecting the splicing of group I or group II mitochondrial introns. Northern blot analysis with 9 different mitochondrial probes, specific for 7 separate transcription units, suggested that the NAM1 protein is not a general mitochondrial transcription factor but that it is selectively and predominantly required for the processing and/or for the stability of tha atp6 transcripts and of 2 intron-containing pre-messenger ribonucleic acids (mRNA).
A fusion plasmid allowing the expression of the NAM1 protein (NAM1p) in Escherichia coli was constructed, after which, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) purification of the resultant protein was used to obtain antibodies which recognised primarily a single protein under Western blot conditions. Submitochondrial localization experiments have shown that NAM1p is a soluble protein of the mitochondrial matrix. It is proposed that NAM1p could be an RNA-convoying protein stabilizing and directing mitochondrial transcripts towards the inner face of the inner membrane where translation and assembly seem to occur.
In order to find other gene products interacting with NAM1, a search was made for genes which, expressed on multicopy plasmids, compensated for the respiratory deficiency of nam1-inactivated strains. A 3.8 kb yeast genomic fragment LM9, carrying such a suppressor gene was isolated from a wild type genomic bank after screening for functional complementation of strains deleted-disrupted in NAM1. Sequencing and subcloning the fragment have shown that an us open reading frame (ORF), encoding a putative protein of 225 amino acids, bears the suppressor activity. The function of that protein is under investigation.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences inorganic chemistry alkali metals
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics mutation
- natural sciences chemical sciences electrochemistry electrophoresis
- natural sciences chemical sciences organic chemistry amines
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
91198 GIF SUR YVETTE
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.