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Chromosome walking in Arabidopsis Thaliana


Arabidopsis has been used as an experimental model system for the study of the physiology, biochemistry, growth and development of a generic flowering plant at the molecular level.
Chromosome walking has been performed on the top halves of Arabidopsis chromosomes 4 and 5, using yeast artificial chromosome (YAC) clones from 3 different YAC libraries. All the restriction fragment length polymorphism (RFLP) markers available have been used to hybridize the YAC libraries and, once all the mapped markers has corresponding YAC clones, then walks were started.
A specific region of chromosome 4 has been studied using the RFLP markers 210, 326, 580, 226, 4108, 10086, 6837 and 4546. High density colony hybridization is performed and positive colonies are confirmed by Southern blot analysis.


Norwich Research Park, Colney
United Kingdom