Objective
In the near future foods produced by means of genetic engineering will be increasingly commercially available. On the other hand the EU Regulation No. 258/97 on Novel Foods and Novel Food Ingredients requires labelling of such foods. Thus validated methods to identify genetically modified foods must be available to the food control. The analytical techniques applied within the scope of the project are predominantly based on PCR (polymerase chain reaction) and hybridization techniques using specific probes in different variations. But also alternative approaches to DNA diagnostic methods - i.e. immunochemical protein analysis - are applied.
The project aims to develop and evaluate methods for the identification of foods produced by genetic engineering and to provide a data bank. Six methods will be choosen for intercomparison studies. These methods shall be internationalized to facilitate the exchange of products and to support the harmonization of labelling practice. Therefore methods succesfully applied in ringtesting studies will be proposed for conversion into CEN standards.
A variety of DNA extraction methods were developed for raw and processed foods. Many primer pairs have been deduced and were used to test the quality of extracted DNA for the PCR. Extraction methods and deduced primer were compared between partners so that on the basis of these results ringtesting of elevated methods can start during the second year of the project. Methods were developed to identify kanamycin (PCR) and hygromycin resistance (PCR ELISA) in transgenic plants. NASBA and 3SR techniques have been initiated in microorganisms. AFLP fingerprinting technique was applied succesfully with DNA isolated from dried rice grains. The structure of a data bank was developed so that parallely a modul system comprising of proposals for DNA-extraction, primer, probes and PCR-condition for a given genetically modified food can now be build up.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- medical and health sciences medical biotechnology genetic engineering
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences microbiology
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds cereals
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
14191 Berlin
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.