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Community method for the detection and diagnosis of potato brown rot - pseudomonas solanacearum

Objective



The major aim is to provide a harmonised scheme for the detection and diagnosis of Pseudomonas (Ratstonia) solanacearum, the potato brown rot pathogen which will be used to support the proposed EC Potato Brown Rot Control Directive.

Potato brown rot disease has recently become established in several Member States and poses a major risk to potato production in the EU, affecting the seed potato, ware potato, and potato processing industries.

The success of implementation of the Control Directive depends on having reliable diagnostic methods with adequate sensitivity and specificity. Because of its recent establishment in the EU, such tried and tested methods are not yet available.

Early work will develop and evaluate a series of methods with potential for detection of the pathogen in all the substrates covered in the Control Directive. These include potato, tomato and weeds (biotic substrates) and soil, irrigation and surface water, potato waste and sewage (abiotic substrates). Much of this early development will be on sample preparation rather than on the methods themselves, because we have methods which work quite well on pure cultures of the bacterium but not, for example, in potato tissue, soil or water.

The methods with the best potential for each substrate will be selected and further refined if necessary. These methods will then be evaluated for each substrate in over 30 laboratories throughout the EU. Parallel but smaller studies will evaluate methods for identifying and confirming cultures of the pathogen, also a process demanded by the Control Directive.
Another small study will develop and evaluate kits for rapid detection of the pathogen in symptomatic plant tissues, which will be of use during statutory inspections especially of imports from known infected third countries.
A final scheme will then be agreed to cover all detection and diagnostic requirements of the Directive.

A series of deliverables will include a video of practical details of the agreed scheme, sets of photographic slides of symptoms and test results, standard operating procedures for all methods in the final scheme and various reference materials for use with the scheme (antiserum, cultures, reagents).

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

THE MINISTER OF AGRICULTURE, FISHERIES AND FOOD
Address
Sand Hutton
YO41 1LZ York
United Kingdom

Participants (9)

Biologische Bundesanstalt für Land- und Forstwirtschaft
Germany
Address
81,Stahnsdorfer Damm
14532 Kleinmachonow
Direccao-General de Protecçao das Culturas
Portugal
Address
Tapada Da Ajuda
1300 Lisboa
Federal Office and Research Centre ofAgriculture
Austria
Address
191,Spargelfeldstrasse
1226 Wien
Fédération Régionale des Groupements de Défense contre les Ennemis des Culture
France
Address
10,Rue Le Nôtre
49044 Angers
INSTITUTO VALENCIANO DE INVESTIGACIONES AGRARIAS
Spain
Address
Km 4.5,Ctra. Moncada-naquera Km 4.5
46113 Moncada (Valencia)
Ministerie van Landbouw, Natuurbeheeren Visserij
Netherlands
Address
15,Geertjesweg
6700 HC Wageningen
Rijkscentrum voor Landbouwkundig Onderzoek - Research Organisation
Belgium
Address
96,Burg. Van Gansberghelaan
9820 Merelbeke
The Swedish University of Agricultural Sciences
Sweden
Address

750 07 Uppsala
Università degli Studi di Bologna
Italy
Address
Via Filippo Re 7
40126 Bologna