The objective is to develop and apply analytical methods for the identification of populations and individuals exposed to selected pesticides and to evaluate levels of exposure.
This project deals with the development and application of analytical methods to identify people exposed to pesticides (professional exposure) and the evaluation of the levels of their exposures. A range of methodologies, both biological and chemical, will be utilized in order to evaluate the most adequate techniques to be applied in relation to pesticide residues or to their metabolite residues.
Exposed professional groups are identified and analyzed in the regions of Campania (Italy), Liguria (Italy), Maresme (Spain), and Helsinki (Finland). The techniques to be applied or developed, are the following.
Biological methods: chromosome aberrations (CA); sister chromatid exchanges (SCE); micronuclei (MN); poly(adenosine diphosphate (ADP) ribose) polymerase (POL); urine analyses (UA); and cholinesterase.
Chemical methods: deoxyribonucleic acid (DNA) adducts; haemoglobin amino acids adducts; and plasma proteins adducts.
All these techniques are also applied to the model animals groups exposed to pesticides, on which the same effects and the metabolism of some pesticides will be investigated.
Pesticides to be analyzed are; thiocarbamates, dibromoethane, dichloroethane, 1,3-dichloropropene, methylisothiocyanate, methylbromide, atrazine, molinate, organophosphate insecticides, alachlor, Chlordimeform, vinclozolin, amitrole, ethylbromide, ethylene oxide, and methyl parathion. For each one of these pesticides a review of their known toxicological potential will be prepared.
Samples from a group of around 3000 unexposed people already participating in a large biomonitoring programme undertaken during the last 5 years for clinical end points will be analyzed as controls.
Funding SchemeCSC - Cost-sharing contracts
106 91 Stockholm