Objective The study will test the existing hypotheses of the molecular-genetic basis of chloroquine-resistance using resistance-diagnostic techniques simply carried out on blood spots collected in the field. Control studies will be carried out on continuous and clones from field isolates. The drug sensitivity of falciparum malaria in patients in Guinea-Bissau and in Indonesia will be tested in vivo and in vitro. Blood spots on glass fibre discs and cryopreserved isolates will be collected. Diagnostic probe and polymerase chain reaction (PCR) studies will be carried out on the blood spots to characterize resistance-related genes. The cryopreserved material will be recovered into continuous cultures and clones selected. Probe, PCR and restriction studies will be carried out on the clones, together with drugsensitivity tests for comparison with results obtained on the fresh isolates. Knowledge of the way in which malaria parasites become resistant will give insights into the mode of action of drugs and enable the development of improved and novel drug entities to attack morbidity and mortality due to the disease. Fields of science medical and health scienceshealth sciencesinfectious diseasesmalariasocial sciencessociologydemographymortalitymedical and health scienceshealth sciencespublic healthepidemiologymedical and health sciencesclinical medicineallergologydrug allergymedical and health sciencesbasic medicinepharmacology and pharmacydrug resistance Programme(s) FP3-STD 3 - Specific research and technological development programme (EEC) in the field of the life sciences and technologies for developing countries, 1990-1994 Topic(s) Data not available Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator London School of Hygiene and Tropical Medicine Address Keppel street WC1E 7HT London United Kingdom See on map EU contribution € 0,00 Participants (4) Sort alphabetically Sort by EU Contribution Expand all Collapse all Dr. Sardjito's General Hospital Indonesia EU contribution € 0,00 Address 55281 Yogyakarta See on map LEIDEN UNIVERSITY Netherlands EU contribution € 0,00 Address 62,wassenaarseweg 62 2300 RC Leiden See on map Ministry of Public Health Guinea-Bissau Guinea-Bissau EU contribution € 0,00 Address Bissau See on map UNIVERSIDADE NOVA DE LISBOA Portugal EU contribution € 0,00 Address Rua de junqueira 96 1349 Lisboa See on map
