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Content archived on 2024-04-19

Epidemiology of drug-resistance in plasmodium falciparum

Objective



The study will test the existing hypotheses of the molecular-genetic basis of chloroquine-resistance using resistance-diagnostic techniques simply carried out on blood spots collected in the field. Control studies will be carried out on continuous and clones from field isolates.

The drug sensitivity of falciparum malaria in patients in Guinea-Bissau and in Indonesia will be tested in vivo and in vitro. Blood spots on glass fibre discs and cryopreserved isolates will be collected.

Diagnostic probe and polymerase chain reaction (PCR) studies will be carried out on the blood spots to characterize resistance-related genes. The cryopreserved material will be recovered into continuous cultures and clones selected. Probe, PCR and restriction studies will be carried out on the clones, together with drugsensitivity tests for comparison with results obtained on the fresh isolates.

Knowledge of the way in which malaria parasites become resistant will give insights into the mode of action of drugs and enable the development of improved and novel drug entities to attack morbidity and mortality due to the disease.

Fields of science (EuroSciVoc)

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Topic(s)

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Call for proposal

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Funding Scheme

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CSC - Cost-sharing contracts

Coordinator

London School of Hygiene and Tropical Medicine
EU contribution
No data
Address
Keppel Street
WC1E 7HT London
United Kingdom

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Total cost

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Participants (4)

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