Objective Embryonic stem (ES) cells have the potential to differentiate into any type of cell as well as to renew indefinitely in culture. They hold great potential for the development of personalized medicines. However, the molecular mechanisms underpinning cell fate decisions by individual cells are poorly understood. There is compelling evidence that two large multi-protein machines, the Nucleosome Remodelling and Deacetylation (NuRD) complex and the Polycomb Repressive Complexes (PRCs), modulate chromatin structure to control stem cell renewal, lineage commitment and differentiation. Moreover, they are clearly implicated in cancer.The goal of 4DCellFate is to understand how the PRC/NuRD complexes and their plethora of interactions (protein/protein, protein/nucleosome, protein/nucleic acids) regulate cell fate. To obtain this global, quantitative and dynamic – 4D – understanding of the structure/functions of these two multi-protein machines during ES cell differentiation and in different disease states, we propose a large scale multi-disciplinary “data-gathering” approach combining European excellence in interactomics (affinity purification, quantitative mass spectrometry-based proteomics, ChIP-seq analysis, light microscopy), structural biology (X-ray crystallography, NMR, native-state mass spectrometry, Electron Microscopy, biochemistry/biophysics), cellular, tumour, and computational biology. We expect to significantly advance the technology, mainly in the fields of proteomics, structural biology, and small molecule screening, and to make our knowledge, reagents and data publicly available to the scientific community.The ultimate outcome of 4DCellFate will be to lay the foundations for understanding the role of the PRC/NuRD complexes in ES cell differentiation and cancer, specifically in leukaemogenesis. Three SMEs and one large multi-national pharma will be actively involved ensuring that our findings can be translated into new ways to control complex diseases. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesphysical sciencesopticsmicroscopyelectron microscopymedical and health sciencesmedical biotechnologycells technologiesstem cellsnatural sciencesbiological sciencesmolecular biologystructural biology Programme(s) FP7-HEALTH - Specific Programme "Cooperation": Health Topic(s) HEALTH.2011.2.1.1-2 - Proteins and their interactions in health and disease Call for proposal FP7-HEALTH-2011-two-stage See other projects for this call Funding Scheme CP-IP - Large-scale integrating project Coordinator FUNDACIO CENTRE DE REGULACIO GENOMICA EU contribution € 2 935 357,20 Address CARRER DOCTOR AIGUADER 88 08003 Barcelona Spain See on map Region Este Cataluña Barcelona Activity type Research Organisations Administrative Contact Mariana Morlans (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Participants (12) Sort alphabetically Sort by EU Contribution Expand all Collapse all THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE United Kingdom EU contribution € 3 184 984,40 Address TRINITY LANE THE OLD SCHOOLS CB2 1TN Cambridge See on map Region East of England East Anglia Cambridgeshire CC Activity type Higher or Secondary Education Establishments Administrative Contact Renata Schaeffer (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data KOBENHAVNS UNIVERSITET Denmark EU contribution € 804 300,00 Address NORREGADE 10 1165 Kobenhavn See on map Region Danmark Hovedstaden Byen København Activity type Higher or Secondary Education Establishments Administrative Contact Ivan Kristoffersen (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data UNIVERSITAIR MEDISCH CENTRUM UTRECHT Participation ended Netherlands EU contribution € 135 392,79 Address HEIDELBERGLAAN 100 3584 CX Utrecht See on map Region West-Nederland Utrecht Utrecht Activity type Higher or Secondary Education Establishments Administrative Contact E.Th. 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Antwerpen Activity type Higher or Secondary Education Establishments Administrative Contact Anne Adams (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data EUROPEAN MOLECULAR BIOLOGY LABORATORY Germany EU contribution € 1 274 530,00 Address Meyerhofstrasse 1 69117 Heidelberg See on map Region Baden-Württemberg Karlsruhe Heidelberg, Stadtkreis Activity type Research Organisations Administrative Contact Genevieve Reinke (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV Germany EU contribution € 779 703,00 Address HOFGARTENSTRASSE 8 80539 Munchen See on map Region Bayern Oberbayern München, Kreisfreie Stadt Activity type Research Organisations Administrative Contact Ulrike Jendis (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data ISTITUTO EUROPEO DI ONCOLOGIA SRL Italy EU contribution € 622 877,60 Address VIA FILODRAMMATICI 10 20121 Milano See on map Region Nord-Ovest Lombardia Milano Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Ilaria Foti (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Horizon Discovery Limited United Kingdom EU contribution € 379 100,00 Address CAMBRIDGE RESEARCH PARK CB25 9TL Cambridge See on map Region East of England East Anglia Cambridgeshire CC Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Christopher Torrance (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data QIAGEN AARHUS AS Denmark EU contribution € 572 500,00 Address SILKEBORGVEJ 2 8000 Aarhus See on map Region Danmark Midtjylland Østjylland Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Kenneth G. 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United Kingdom EU contribution € 9 000,00 Address Great West Road 980 TW89GS Brentford See on map Region London Outer London — West and North West Hounslow and Richmond upon Thames Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Elaine Irving (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data STICHTING RADBOUD UNIVERSITEIT Netherlands EU contribution € 579 184,01 Address HOUTLAAN 4 6525 XZ Nijmegen See on map Region Oost-Nederland Gelderland Arnhem/Nijmegen Activity type Higher or Secondary Education Establishments Administrative Contact Geurt Van Renselaar (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data