Objectif
We propose a technology that will sit at the front-end of sequencing pipelines, present and future, and will significantly enhance the quality and throughput of DNA sequencing.
Although much attention has been given to throughput/cost of the sequencing process itself, the same cannot be said for the preparation of samples. Identified bottlenecks are (1) sequencing technologies require days of upfront sample preparation which is further increased when sequencing selected parts of the genome; (2) genome assembly relies on computationally intensive comparisons to the reference genome because existing technologies produce short sequence reads; (3) it is difficult to begin with small amounts of sample material comprising micro-biopsies and single cells.
The CELL-O-MATIC project will synergize efforts from SMEs, academics and large companies to address these bottlenecks by developing chip-based systems that process DNA from individual cells, ready for next generation high-throughput sequencing.
Single cell analysis has numerous applications in systems biology but we will emphasize DNA isolation and sequencing from circulating tumor cells (CTC), which have a strong prognostic value in cancer management.
A second innovation will be to develop methods that enable up to whole chromosome lengths of DNA to be contiguously mapped using nanofluidics. The inclusion of nanofluidics makes the project particularly distinctive and introduces European SMEs to an area that so far has been the domain of US companies.
A modular prototype comprising, a chip, fluid and thermal control, sonication and optical detection will be developed. Samples prepared using CELL-O-MATIC technology will be benchmarked in a high throughput environment with samples prepared by existing methods. Finally, the information obtained from the CELL-O-MATIC processed sample material will be validated for its utility as an aid to clinical decision making.
Champ scientifique
Appel à propositions
FP7-HEALTH-2011-two-stage
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Régime de financement
CP-FP - Small or medium-scale focused research projectCoordinateur
2800 Kongens Lyngby
Danemark
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Participants (13)
OX1 2JD Oxford
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5656 AG Eindhoven
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Participation terminée
68305 Mannheim
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BD1 3BN BRADFORD
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4102 Seraing
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OX4 4GA Oxford
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2800 Kongens Lyngby
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02130 Espoo
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94270 Le Kremlin-Bicetre
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1228 PLAN LES OUATES
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WCIN 2EB LONDON
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3450 ALLEROD
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02138 CAMBRIDGE
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