Objetivo Plant (+)strand RNA viruses account for important losses in crop yields worldwide. They complete complex infectious cycles by replicating, moving and inactivating plant defense mechanisms through multiple interactions of their RNA or proteins with host factors. Plants have evolved two main antiviral immunity systems operated by ARGONAUTE (AGO) proteins that target viral RNAs in the RNA silencing pathway or by resistance proteins that recognize viral-encoded proteins through protein-protein interactions. However, neither the identification of AGO target sites in viral RNAs nor the elucidation of the entire repertoire of proteins interacting with viral proteins has been reported for any plant virus. Now, the recent development of new technologies (and associated tools) such as high-throughput DNA sequencing and high performance mass spectrometry (MS) platforms makes possible to address these questions at a genome-wide level. InterAcTEV will combine these new technologies to study the recently described Arabidopsis/Tobacco etch virus (TEV)-TAMPS pathosystem with a multidisciplinary approach aimed to identify AGO target sites in TEV RNAs and to generate the first complete protein-protein interactome map for a plant virus. First, RNA-immunoprecipitation coupled with high-throughput sequencing methods will be optimized to identify AGO target sites in TEV RNAs at a genome-wide level. Second, we will combine novel high-affinity purification methods with MS analyses to identify the specific pool of Arabidopsis proteins interacting with each TEV protein. InterAcTEV will generate key knowledge to open new avenues for the understanding of resistance mechanisms in plants and to develop novel antiviral strategies for crop protection and securement of world’s food supply. Finally, the funding of InterAcTEV will allow the Experienced Researcher to initiate his own research project in the host laboratory and facilitate his transition to an independent research position in Europe. Ámbito científico medical and health scienceshealth sciencesinfectious diseasesRNA virusesnatural sciencesbiological sciencesmicrobiologyvirologynatural sciencesbiological sciencesgeneticsRNAmedical and health sciencesbasic medicinepharmacology and pharmacydrug resistancemultidrug resistancemedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsantivirals Programa(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Tema(s) MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF) Convocatoria de propuestas H2020-MSCA-IF-2014 Consulte otros proyectos de esta convocatoria Régimen de financiación MSCA-IF-EF-RI - RI – Reintegration panel Coordinador AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS Aportación neta de la UEn € 170 121,60 Dirección CALLE SERRANO 117 28006 Madrid España Ver en el mapa Región Comunidad de Madrid Comunidad de Madrid Madrid Tipo de actividad Research Organisations Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 170 121,60