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Implementation of Enzymatic Activity in a Naïve, de novo Designed Protein Scaffold by Rational Design and Laboratory Evolution

Publications

Optimization of Enzyme Mechanism along the Evolutionary Trajectory of a Computationally Designed (Retro-)Aldolase

Author(s): Cathleen Zeymer, Reinhard Zschoche, Donald Hilvert
Published in: Journal of the American Chemical Society, Issue 139/36, 2017, Page(s) 12541-12549, ISSN 0002-7863
DOI: 10.1021/jacs.7b05796

Efficient laboratory evolution of computationally designed enzymes with low starting activities using fluorescence-activated droplet sorting

Author(s): Richard Obexer, Moritz Pott, Cathleen Zeymer, Andrew D. Griffiths, Donald Hilvert
Published in: Protein Engineering Design and Selection, Issue 29/9, 2016, Page(s) 355-366, ISSN 1741-0126
DOI: 10.1093/protein/gzw032